| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76837-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76836-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34951-1mg | |||
| Carrier-Free | NBP2-34951-100ug | |||
| Carrier-Free | NBP2-34951-500ug | |||
![Western Blot: MANF Antibody (1D10) [H00007873-M01] - Western Blot detection against Immunogen (33.33 KDa).](http://images.novusbio.com/fullsize/ARMET-Antibody-1D10-Western-Blot-H00007873-M01-img0002.jpg "Western Blot: MANF Antibody (1D10) [H00007873-M01] - Western Blot detection against Immunogen (33.33 KDa).")
![Immunocytochemistry/Immunofluorescence: MANF Antibody (1D10) [H00007873-M01] - Analysis of monclonal antibody to MANF antibody on HeLa cell. Image from verified customer review.](http://images.novusbio.com/fullsize/MANF-Antibody-1D10-Immunocytochemistry-Immunofluorescence-H00007873-M01-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: MANF Antibody (1D10) [H00007873-M01] - Analysis of monoclonal antibody to MANF on HeLa cell . Antibody concentration 40 ug/ml.](http://images.novusbio.com/fullsize/ARMET-Antibody-1D10-Immunocytochemistry-Immunofluorescence-H00007873-M01-img0004.jpg)
![Sandwich ELISA: MANF Antibody (1D10) [H00007873-M01] - Detection limit for recombinant GST tagged MANF is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ARMET-Antibody-1D10-Sandwich-ELISA-H00007873-M01-img0005.jpg)
![Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF NBP1-76837 (Lane 1: 0.125 ug/mL, Lane 2: 0.25 ug/mL and Lane 3: 0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 55kDa.](http://images.novusbio.com/fullsize/MANF-Antibody-Western-Blot-NBP1-76837-img0005.jpg "Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF NBP1-76837 (Lane 1: 0.125 ug/mL, Lane 2: 0.25 ug/mL and Lane 3: 0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 55kDa.")
![Immunocytochemistry/Immunofluorescence: MANF Antibody [NBP1-76837] - Immunofluorescence of MANF in Human Brain tissue with MANF antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/MANF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76837-img0003.jpg)
![Immunohistochemistry-Paraffin: MANF Antibody [NBP1-76837] - Human brain tissue with ARMET antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/MANF-Antibody-Immunohistochemistry-Paraffin-NBP1-76837-img0002.jpg)
![Western Blot: MANF Antibody [NBP1-76837] - Rat brain tissue lysate with ARMET antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/MANF-Antibody-Western-Blot-NBP1-76837-img0001.jpg)
![Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: MANF NBP1-76837, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/MANF-Antibody-Western-Blot-NBP1-76837-img0004.jpg)
![Western Blot: MANF Antibody - BSA Free [NBP1-76837] - Figure 3 Western Blot Validation in Rat Brain Tissue LysateLoading: 15 u of lysates per lane.Antibodies: MANF (A: 1 u /mL and B: 2 u /mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76837_rabbit-polyclonal-manf-antibody-271120242042538.jpg)
![Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76837] - Figure 5 Immunofluorescence Validation of MANF in Human Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling MANF with at 20 u /mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76837_rabbit-polyclonal-manf-antibody-271120241928819.jpg)
![Western Blot: MANF Antibody - BSA Free [NBP1-76837] - Figure 2 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human MANF recombinant protein per lane.Antibodies: MANF (Lane 1: 0.125 u/mL, Lane 2: 0.25 u/mL and Lane 3: 0.5 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 55kD.](http://images.novusbio.com/fullsize/nbp1-76837_rabbit-polyclonal-manf-antibody-2711202420243364.gif)
![Immunohistochemistry: MANF Antibody - BSA Free [NBP1-76837] - Figure 4 Immunohistochemistry Validation of MANF in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-MANF antibody at 2.5 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76837_rabbit-polyclonal-manf-antibody-271120242004148.jpg)
![Western Blot: MANF Antibody - BSA Free [NBP1-76837] - Figure 2 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human MANF recombinant protein per lane.Antibodies: MANF (Lane 1: 0.125 u/mL, Lane 2: 0.25 u/mL and Lane 3: 0.5 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 55kD.](http://images.novusbio.com/fullsize/nbp1-76837_rabbit-polyclonal-manf-antibody-2711202419241372.gif)
![Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76837] - Figure 5 Immunofluorescence Validation of MANF in Human Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling MANF with at 20 u /mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76837_rabbit-polyclonal-manf-antibody-271120242042593.jpg)
![Immunohistochemistry: MANF Antibody - BSA Free [NBP1-76837] - Figure 4 Immunohistochemistry Validation of MANF in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-MANF antibody at 2.5 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76837_rabbit-polyclonal-manf-antibody-271120242092087.jpg)
![Western Blot: MANF Antibody - BSA Free [NBP1-76837] - Figure 3 Western Blot Validation in Rat Brain Tissue LysateLoading: 15 u of lysates per lane.Antibodies: MANF (A: 1 u /mL and B: 2 u /mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76837_rabbit-polyclonal-manf-antibody-27112024205239.jpg)
![Knockout Validated: MANF Antibody - BSA Free [NBP1-76836] - KO Validation in HEK293T Cells.Loading: 10 ug of HEK293T WT cell lysates or MANF KO cell lysates. Antibodies: MANF (1 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76836_rabbit-polyclonal-manf-antibody-271120241939270.gif "<b>Genetic Strategies Validation. </b>Knockout Validated: MANF Antibody - BSA Free [NBP1-76836] - KO Validation in HEK293T Cells.Loading: 10 ug of HEK293T WT cell lysates or MANF KO cell lysates. Antibodies: MANF (1 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: MANF Antibody [NBP1-76836] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF NBP1-76836 (Lane 1: 0.125 ug /mL, Lane 2: 0.25 ug/mL and Lane 3: 0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 55kDa.](http://images.novusbio.com/fullsize/MANF-Antibody-Western-Blot-NBP1-76836-img0007.jpg)
![Western Blot: MANF Antibody [NBP1-76836] - Western Blot Validation in Human, Mouse (NIH/3T3) and Rat (YB2/0) Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: MANF NBP1-76836 (2 ug /mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/MANF-Antibody-Western-Blot-NBP1-76836-img0006.jpg)
![Western Blot: MANF Antibody - BSA Free [NBP1-76836] - Validation in Human Tissues.Loading: 15 ug of lysates per lane.Antibodies: MANF, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76836_rabbit-polyclonal-manf-antibody-2711202420432342.gif)
![Western Blot: MANF Antibody - BSA Free [NBP1-76836] - Validation in Rat Liver.Loading: 15 ug of lysates per lane.Antibodies: MANF, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 ug/mLLane 2: 2 ug/mLLane 3: 4 ug/mL](http://images.novusbio.com/fullsize/nbp1-76836_rabbit-polyclonal-manf-antibody-2711202419172262.gif)
![Western Blot: MANF Antibody - BSA Free [NBP1-76836] - Validation in Mouse TissuesLoading: 15 ug of lysates per lane.Antibodies: MANF, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76836_rabbit-polyclonal-manf-antibody-2711202419531622.gif)
![Immunohistochemistry: MANF Antibody - BSA Free [NBP1-76836] - Validation of MANF in Human Brain Tissue.Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-MANF antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76836_rabbit-polyclonal-manf-antibody-2711202419261842.gif)
![Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] - Validation of MANF in Rat Liver.Immunofluorescent analysis of 4% paraformaldehyde-fixed rat liver cells labeling MANF with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp1-76836_rabbit-polyclonal-manf-antibody-271120241937747.jpg)
![Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] - Validation of MANF in Mouse Pancreas. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas cells labeling MANF at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp1-76836_rabbit-polyclonal-manf-antibody-2711202420312931.jpg)
![Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] - Validation of MANF in Human Liver.Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver cells labeling MANF at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp1-76836_rabbit-polyclonal-manf-antibody-2711202420414580.jpg)
![Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] - Validation of MANF in Human Colon.Immunofluorescent analysis of 4% paraformaldehyde-fixed human colon cells labeling MANF with at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp1-76836_rabbit-polyclonal-manf-antibody-2711202419392744.jpg)
![Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] - Validation of MANF in Human Cervix.Immunofluorescent analysis of 4% paraformaldehyde-fixed human cervix cells labeling MANF at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp1-76836_rabbit-polyclonal-manf-antibody-2711202419333467.jpg)
![Western Blot: ARMET Antibody [NBP1-90099] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/ARMET-Antibody-Western-Blot-NBP1-90099-img0006.jpg "Western Blot: ARMET Antibody [NBP1-90099] - Analysis in human cell line HepG2.")
![Immunocytochemistry/Immunofluorescence: ARMET Antibody [NBP1-90099] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ARMET-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90099-img0007.jpg)
![Immunohistochemistry-Paraffin: ARMET Antibody [NBP1-90099] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ARMET-Antibody-Immunohistochemistry-Paraffin-NBP1-90099-img0012.jpg)
![Immunohistochemistry-Paraffin: ARMET Antibody [NBP1-90099] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/ARMET-Antibody-Immunohistochemistry-Paraffin-NBP1-90099-img0009.jpg)
![Immunohistochemistry-Paraffin: ARMET Antibody [NBP1-90099] - Staining of human rectum shows moderate cytoplasmic positivity in peripheral ganglia, as well as glandular and lymphoid cells.](http://images.novusbio.com/fullsize/ARMET-Antibody-Immunohistochemistry-Paraffin-NBP1-90099-img0010.jpg)
![Immunohistochemistry-Paraffin: ARMET Antibody [NBP1-90099] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/ARMET-Antibody-Immunohistochemistry-Paraffin-NBP1-90099-img0011.jpg)
![SDS-Page: Human MANF Protein [NBP2-34951]](http://images.novusbio.com/fullsize/Human-MANF-Protein-SDS-Page-NBP2-34951-img0001.jpg "SDS-Page: Human MANF Protein [NBP2-34951]")
![SDS-Page: Recombinant Mouse MANF Protein [NBP2-61337]](http://images.novusbio.com/fullsize/Recombinant-Mouse-MANF-Protein-SDS-Page-NBP2-61337-img0002.jpg "SDS-Page: Recombinant Mouse MANF Protein [NBP2-61337]")
![Western Blot: MANF Antibody [NBP1-32037] - ARMET Antibody [NBP1-32037] - Sample(30 ug of whole cell lysate) A:293T 12% SDS PAGE, antibody diluted at 1:1500.](http://images.novusbio.com/fullsize/ARMET-Antibody-Western-Blot-NBP1-32037-img0003.jpg "Western Blot: MANF Antibody [NBP1-32037] - ARMET Antibody [NBP1-32037] - Sample(30 ug of whole cell lysate) A:293T 12% SDS PAGE, antibody diluted at 1:1500.")
![Immunocytochemistry/Immunofluorescence: ARMET Antibody [NBP1-32037] - Analysis of methanol-fixed HepG2, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/ARMET-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32037-img0002.jpg)
![Western Blot: ARMET Antibody [NBP1-06020] - Analysis of ARMET using this antibody at 0.3 ug/ml in Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ARMET-Antibody-Western-Blot-NBP1-06020-img0002.jpg "Western Blot: ARMET Antibody [NBP1-06020] - Analysis of ARMET using this antibody at 0.3 ug/ml in Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: MANF Antibody [NBP2-99774] - Immunofluorescence staining of MANF in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MANF polyclonal antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/MANF-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99774-img0001.jpg "Immunocytochemistry/Immunofluorescence: MANF Antibody [NBP2-99774] - Immunofluorescence staining of MANF in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MANF polyclonal antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: MANF Antibody [NBP2-99774] - Immunochemical staining of human MANF in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MANF-Antibody-Immunohistochemistry-Paraffin-NBP2-99774-img0002.jpg)
![Western Blot: MANF Antibody [NBP2-99775] - Anti-MANF rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 18 kDa](http://images.novusbio.com/fullsize/MANF-Antibody-Western-Blot-NBP2-99775-img0002.jpg "Western Blot: MANF Antibody [NBP2-99775] - Anti-MANF rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 18 kDa")
![Immunoprecipitation: MANF Antibody [NBP2-99775] - MANF was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate 4ul anti-MANF rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MANF rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 17 kDa](http://images.novusbio.com/fullsize/MANF-Antibody-Immunoprecipitation-NBP2-99775-img0001.jpg)