![Western Blot: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. Lane 1: Mouse brain(no heat) tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat brain(no heat) tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32557) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-278202416285214.jpg "Western Blot: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. Lane 1: Mouse brain(no heat) tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat brain(no heat) tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32557) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | PSH02-41 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within human MAG/Siglec-4a aa 1- 536 / 626. (Uniprot: P20916) |
| Localization | Cell membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MAG |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 69 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for MAG/Siglec-4a Antibody (NBP3-32557)Find related products by research area.
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| Gene Symbol | MAG |
![Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunofluorescence analysis of paraffin-embedded rat cerebellum tissue labeling MAG/Siglec-4a with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32557, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-29820241435217.jpg "Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunofluorescence analysis of paraffin-embedded rat cerebellum tissue labeling MAG/Siglec-4a with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32557, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32557) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-298202414115423.jpg "Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32557) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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