![Western Blot: Lysyl tRNA synthetase Antibody [NB100-1719] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-Lysyl tRNA synthetase antibody NB100-1719 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).](http://images.novusbio.com/fullsize/Lysyl-tRNA-synthetase-Antibody-Western-Blot-NB100-1719-img0002.jpg "Western Blot: Lysyl tRNA synthetase Antibody [NB100-1719] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-Lysyl tRNA synthetase antibody NB100-1719 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 550 and the C-terminus (residue 597) of human Lysyl-tRNA Synthetase using the numbering given in entry NP_005539.1 (GeneID 3735). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KARS1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry 1:100 to 1:500. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue section |
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| Control |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KARS1 |
| Entrez |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: Lysyl tRNA synthetase Antibody [NB100-1719] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Lysyl tRNA synthetase used at a dilution of 1:100.](http://images.novusbio.com/fullsize/Lysyl-tRNA-synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1719-img0004.jpg "Immunocytochemistry/Immunofluorescence: Lysyl tRNA synthetase Antibody [NB100-1719] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Lysyl tRNA synthetase used at a dilution of 1:100.")
![Immunohistochemistry: Lysyl tRNA synthetase Antibody [NB100-1719] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Lysyl-tRNA-synthetase-Antibody-Immunohistochemistry-NB100-1719-img0007.jpg "Immunohistochemistry: Lysyl tRNA synthetase Antibody [NB100-1719] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1ug/ml). Detection: DAB")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")