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Lysine Hydroxylase 2/PLOD2 Antibody (OTI6D1) - Azide and BSA Free

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Western Blot: Lysine Hydroxylase 2/PLOD2 Antibody (OTI6D1) - Azide and BSA Free [NBP2-72556] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLOD2.
Western Blot: Lysine Hydroxylase 2/PLOD2 Antibody (OTI6D1) - Azide and BSA Free [NBP2-72556] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLOD2.
Immunohistochemistry: Lysine Hydroxylase 2/PLOD2 Antibody (OTI6D1) - Azide and BSA Free [NBP2-72556] - Analysis of Human Kidney tissue.
Immunohistochemistry: Lysine Hydroxylase 2/PLOD2 Antibody (OTI6D1) - Azide and BSA Free [NBP2-72556] - Analysis of Adenocarcinoma of Human colon tissue.
Immunohistochemistry: Lysine Hydroxylase 2/PLOD2 Antibody (OTI6D1) - Azide and BSA Free [NBP2-72556] - Analysis of Human prostate tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI6D1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72556AF350
Alexa Fluor 405
NBP2-72556AF405
Alexa Fluor 488
NBP2-72556AF488
Alexa Fluor 532
NBP2-72556AF532
Alexa Fluor 594
NBP2-72556AF594
Alexa Fluor 647
NBP2-72556AF647
Alexa Fluor 700
NBP2-72556AF700
Alexa Fluor 750
NBP2-72556AF750
Allophycocyanin
NBP2-72556APC
Biotin
NBP2-72556B
CoraFluor 1
NBP2-72556CL1
DyLight 350
NBP2-72556UV
DyLight 405
NBP2-72556V
DyLight 488
NBP2-72556G
DyLight 550
NBP2-72556R
DyLight 594
NBP2-72556DL594
DyLight 650
NBP2-72556C
DyLight 680
NBP2-72556FR
DyLight 755
NBP2-72556IR
FITC
NBP2-72556F
HRP
NBP2-72556H
Janelia Fluor 525
NBP2-72556JF525
Janelia Fluor 585
NBP2-72556JF585
Janelia Fluor 635
NBP2-72556JF635
Janelia Fluor 669
NBP2-72556JF669
PE
NBP2-72556PE
PerCP
NBP2-72556PCP
Unconjugated
NBP2-46451
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46451
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Lysine Hydroxylase 2/PLOD2 Antibody (OTI6D1) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 359-640 of human PLOD2 (NP_891988) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PLOD2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Lysine Hydroxylase 2/PLOD2 Antibody (OTI6D1) - Azide and BSA Free

  • 2-oxoglutarate 5-dioxygenase (lysine hydroxylase) 2
  • 2-oxoglutarate 5-dioxygenase 2
  • LH2
  • Lysine Hydroxylase 2
  • PLOD2
  • procollagen-lysine
  • telopeptide lysyl hydroxylase
  • TLH

Background

PLOD2 is encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PLOD2