![Immunocytochemistry/Immunofluorescence: Lyp/PTPN22 Antibody [NBP2-69989] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69989-img0001.jpg "Immunocytochemistry/Immunofluorescence: Lyp/PTPN22 Antibody [NBP2-69989] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence CPPNKPAESVQSNNS, from the internal region of the protein sequence according to NP_057051; NP_036543.4; NP_001180360.1; NP_001295226.1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | PTPN22 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH 7.3), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Lyp/PTPN22 Antibody (NBP2-69989)Find related products by research area.
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| Gene Symbol | PTPN22 |
![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP2-69989-img0005.jpg "Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Flow Cytometry: Lyp/PTPN22 Antibody [NBP2-69989] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Flow-Cytometry-NBP2-69989-img0002.jpg "Flow Cytometry: Lyp/PTPN22 Antibody [NBP2-69989] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP2-69989-img0003.jpg "Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP2-69989-img0004.jpg "Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
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