Novus Biologicals products are now on bio-techne.com

LMO7 Antibody

Images

 
Orthogonal Strategies: Western Blot: LMO7 Antibody [NBP1-83915] - Analysis in human cell lines HeLa and HEK293 using anti-LMO7 antibody. Corresponding LMO7 RNA-seq data are presented for the same cell lines. ...read more
Immunocytochemistry/ Immunofluorescence: LMO7 Antibody [NBP1-83915] - Staining of human cell line A-431 shows localization to cytosol & actin filaments. Antibody staining is shown in green.
Immunohistochemistry: LMO7 Antibody [NBP1-83915] - LMO7 staining in Porcine Cardiac Tissue: No Primary Control, Isotype IgG Control, LMO7 1:500 dilution (Image 1 and 2). IHC images submitted by a verified customer ...read more
Immunohistochemistry-Paraffin: LMO7 Antibody [NBP1-83915] - Staining of human uterine cervix shows moderate nuclear and membranous positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: LMO7 Antibody [NBP1-83915] - Staining of human duodenum shows both membranous and nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: LMO7 Antibody [NBP1-83915] - Staining of human skeletal muscle shows moderate nuclear and cytoplasmic positivity in striated muscle fibers.
Immunohistochemistry-Paraffin: LMO7 Antibody [NBP1-83915] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, PoSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

LMO7 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KIYGENGSKSMSDVSAEDVQNLRQLRYEEMQKIKSQLKEQDQKWQDDLAKWKDRRKSYTSDLQKKKEEREEIEKQALEKSKRSSKTFKEMLQDRESQNQKSTVPSRRRMYSFDDVLEEGKRPPTMTVSEASYQSERVEEKGATY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LMO7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
LMO7 Protein (NBP1-83915PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-83915 in the following applications:

Publications
Read Publications using NBP1-83915.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (83%). Reactivity reported in scientific literature (PMID: 22253135). Porcine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for LMO7 Antibody

  • FBX20F-box only protein 20
  • KIAA0858FBXO20F-box protein Fbx20
  • LIM domain 7
  • LIM domain only 7
  • LIM domain only protein 7
  • LMO-7
  • LOMP
  • zinc-finger domain-containing protein

Background

LMO7 contains a calponin homology (CH) domain, a PDZ domain, and a LIM domain. An F-box (FBX) domain is present in alternative splice variants. Members of the LIM protein family carry the LIM domain, a unique cysteine-rich zinc-binding domain. Members of the FBX protein family are involved in protein-protein interactions. The encoded protein may be involved in protein-protein interactions. Multiple alternative splice variants have been described but their full-length sequences have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-84843
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87692
Species: Ha, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP1-80833
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF748
Species: Hu, Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, ICC, IHC, Simple Western, WB
AF7829
Species: Hu
Applications: ICC, IHC, WB
H00011155-M06
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IP, KD, WB
NBP1-84443
Species: Hu
Applications: IHC, IHC-P, WB
NLS54
Species: Ca, Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-84841
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-86037
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-15723
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-74462
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC/IF, WB
NBP3-25358
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
DPI00
Species: Hu
Applications: ELISA
NB100-464
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP1-26650
Species: Hu, Mu
Applications: IHC, IP, WB
NBP1-87745
Species: Hu, Rt
Applications: IHC, IHC-P, WB
NBP2-92749
Species: Hu, Mu
Applications: WB
NBP2-25151
Species: Hu, Pm, Mu, Rt
Applications: ICC/IF, WB
NBP1-83915
Species: Hu, Po
Applications: WB, ICC/IF, IHC

Publications for LMO7 Antibody (NBP1-83915)(2)

Review for LMO7 Antibody (NBP1-83915) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other and Porcine.

Reviews using NBP1-83915:
Filter by Applications
IHC
(1)
All Applications
Filter by Species
Other and Porcine
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IHC Other and Porcine 10/08/2021
View

Summary

ApplicationImmunohistochemistry
Sample Testedcardiac tissue
SpeciesOther and Porcine
Lot000004278
CommentsLMO7 staining in Pig Cardiac Tissue

Comments

CommentsStaining was optimized in porcine cardiac tissue.
Dewax, Reydrate and Antigen Retrieval= 1X Trilogy solution (Cell Marque)
Peroxidase Block = 3% H202
Primary Concentration = 1:500
Wash Buffer = 1X TBS -0.1%Tween
Block Buffer = Dako Serum Free Protein Block
Detection= EnVision Detection Systems Peroxidase/DAB, HRP.
***Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LMO7 Antibody (NBP1-83915) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Verified Customer
10/08/2021
Application: IHC
Species: Other and Porcine

Bioinformatics

Gene Symbol LMO7