Orthogonal Strategies: Western Blot: LMO7 Antibody [NBP1-83915] - Analysis in human cell lines HeLa and HEK293 using anti-LMO7 antibody. Corresponding LMO7 RNA-seq data are presented for the same cell lines. ...read more
Immunocytochemistry/ Immunofluorescence: LMO7 Antibody [NBP1-83915] - Staining of human cell line A-431 shows localization to cytosol & actin filaments. Antibody staining is shown in green.
Immunohistochemistry: LMO7 Antibody [NBP1-83915] - LMO7 staining in Porcine Cardiac Tissue: No Primary Control, Isotype IgG Control, LMO7 1:500 dilution (Image 1 and 2). IHC images submitted by a verified customer ...read more
Immunohistochemistry-Paraffin: LMO7 Antibody [NBP1-83915] - Staining of human uterine cervix shows moderate nuclear and membranous positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: LMO7 Antibody [NBP1-83915] - Staining of human duodenum shows both membranous and nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: LMO7 Antibody [NBP1-83915] - Staining of human skeletal muscle shows moderate nuclear and cytoplasmic positivity in striated muscle fibers.
Immunohistochemistry-Paraffin: LMO7 Antibody [NBP1-83915] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
This antibody was developed against Recombinant Protein corresponding to amino acids: KIYGENGSKSMSDVSAEDVQNLRQLRYEEMQKIKSQLKEQDQKWQDDLAKWKDRRKSYTSDLQKKKEEREEIEKQALEKSKRSSKTFKEMLQDRESQNQKSTVPSRRRMYSFDDVLEEGKRPPTMTVSEASYQSERVEEKGATY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LMO7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (83%). Reactivity reported in scientific literature (PMID: 22253135). Porcine reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for LMO7 Antibody
FBX20F-box only protein 20
KIAA0858FBXO20F-box protein Fbx20
LIM domain 7
LIM domain only 7
LIM domain only protein 7
LMO-7
LOMP
zinc-finger domain-containing protein
Background
LMO7 contains a calponin homology (CH) domain, a PDZ domain, and a LIM domain. An F-box (FBX) domain is present in alternative splice variants. Members of the LIM protein family carry the LIM domain, a unique cysteine-rich zinc-binding domain. Members of the FBX protein family are involved in protein-protein interactions. The encoded protein may be involved in protein-protein interactions. Multiple alternative splice variants have been described but their full-length sequences have not been determined.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
We have 1 review tested in 1 species: Other and Porcine.
Reviews using NBP1-83915:
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IHC
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Other and Porcine
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Images
Ratings
Applications
Species
Date
Details
4
reviewed by: Verified Customer
IHC
Other and Porcine
10/08/2021
View
Summary
Application
Immunohistochemistry
Sample Tested
cardiac tissue
Species
Other and Porcine
Lot
000004278
Comments
LMO7 staining in Pig Cardiac Tissue
Comments
Comments
Staining was optimized in porcine cardiac tissue. Dewax, Reydrate and Antigen Retrieval= 1X Trilogy solution (Cell Marque) Peroxidase Block = 3% H202 Primary Concentration = 1:500 Wash Buffer = 1X TBS -0.1%Tween Block Buffer = Dako Serum Free Protein Block Detection= EnVision Detection Systems Peroxidase/DAB, HRP. ***Novus Innovators Program - new species or application used on a primary antibody.
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