LIX Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

LIX ( Liposaccharide-Induced CXC chemokine; also GARG-8 and Cxcl5) is a secreted 8-9 kDa member of the Intercrine alpha (or CxC) family of chemokines. It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium. As a chemokine, LIX demonstrates chemokinetic properties. It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages. Notably, circulating LIX is not derived from fibroblasts, but platelets. This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX. Mouse LIX is synthesized as a 132 amino acid (aa) precursor that contains a 40 aa signal sequence, a 78 aa mature region (aa 41-118), and a cleavable 14 aa C-terminus. The mature region possesses an ELR/GluLeuArg motif between aa 50-52, and an alpha -family characteristic CxC motif between aa 53-55. Although there are no known splice variants of mouse LIX, considerable proteolytic processing occurs at both the N- and C-termini over aa 41-132. This may reduce the MW in SDS-PAGE by as much as 3 kDa. The majority of LIX appears to start between aa 47-50, and this is positively correlated with bioactivity. Over aa 41-118, mouse LIX shares 73% aa sequence identity with rat LIX. Although not a strict ortholog, mouse LIX shares 63% aa sequence identity with human GCP-2.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow Cytometry, IHC-P, Neutralization, Western Blot.

Showing Publications 1 - 5 of 5.

Publications using AF433	Applications	Species
Theivanthiran B, Yarla N, Haykal T et al. Tumor-intrinsic NLRP3-HSP70-TLR4 axis drives premetastatic niche development and hyperprogression during anti-PD-1 immunotherapy Science Translational Medicine 11/23/2022 [PMID: 36417489]
B Theivanthi, KS Evans, NC DeVito, MP Plebanek, M Sturdivant, LP Wachsmuth, AK Salama, Y Kang, D Hsu, JM Balko, DB Johnson, M Starr, AB Nixon, A Holtzhause, BA Hanks A tumor-intrinsic PD-L1-NLRP3 inflammasome signaling pathway drives resistance to anti-PD-1 immunotherapy J. Clin. Invest., 2020-05-01;0(0):. 2020-05-01 [PMID: 32017708] (Western Blot, Mouse)	Western Blot	Mouse
Nikota J, Shen P, Morissette M, Fernandes K, Roos A, Chu D, Barra N, Iwakura Y, Kolbeck R, Humbles A, Stampfli M Cigarette smoke primes the pulmonary environment to IL-1alpha/CXCR-2-dependent nontypeable Haemophilus influenzae-exacerbated neutrophilia in mice. J Immunol, 2014-08-04;193(6):3134-45. 2014-08-04 [PMID: 25092891] (IHC-P, Mouse)	IHC-P	Mouse
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production. Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9. 2009-03-16 [PMID: 19289819] (Neutralization, Mouse)	Neutralization	Mouse

Array AF433

• LIX Antibodies
• LIX Proteins and Enzymes
• LIX ELISAs