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Livin Antibody (OTI1D12)

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Western Blot: Livin Antibody (1D12) [NBP2-02177] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Livin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Livin.
Western Blot: Livin Antibody (1D12) [NBP2-02177] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Livin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: Livin Antibody (1D12) [NBP2-02177] Staining of paraffin-embedded liver tissue using anti-Livin mouse monoclonal antibody.
Flow Cytometry: Livin Antibody (1D12) [NBP2-02177] - Analysis of Jurkat cells, using anti-Livin antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: Livin Antibody (1D12) [NBP2-02177] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-Livinmouse monoclonal antibody.
Flow Cytometry: Livin Antibody (1D12) [NBP2-02177] - Analysis of Hela cells, using anti-Livin antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI1D12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.36 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72206AF350
Alexa Fluor 405
NBP2-72206AF405
Alexa Fluor 488
NBP2-72206AF488
Alexa Fluor 532
NBP2-72206AF532
Alexa Fluor 594
NBP2-72206AF594
Alexa Fluor 647
NBP2-72206AF647
Alexa Fluor 700
NBP2-72206AF700
Alexa Fluor 750
NBP2-72206AF750
Allophycocyanin
NBP2-72206APC
Allophycocyanin/Cy7
NBP2-72206APCCY7
Biotin
NBP2-72206B
CoraFluor 1
NBP2-72206CL1
DyLight 350
NBP2-72206UV
DyLight 405
NBP2-72206V
DyLight 488
NBP2-72206G
DyLight 550
NBP2-72206R
DyLight 594
NBP2-72206DL594
DyLight 650
NBP2-72206C
DyLight 680
NBP2-72206FR
DyLight 755
NBP2-72206IR
FITC
NBP2-72206F
HRP
NBP2-72206H
Janelia Fluor 525
NBP2-72206JF525
Janelia Fluor 549
NBP2-72206JF549
Janelia Fluor 646
NBP2-72206JF646
Janelia Fluor 669
NBP2-72206JF669
PE
NBP2-72206PE
PE/Atto594
NBP2-72206PEATT594
PE/Cy5.5
NBP2-72206PECY55
PE/Cy7
NBP2-72206PECY7
PerCP
NBP2-72206PCP
mFluor Violet 450 SE
NBP2-72206MFV450
mFluor Violet 500 SE
NBP2-72206MFV500
mFluor Violet 610 SE
NBP2-72206MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-72206
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Livin Antibody (OTI1D12) Summary

Immunogen
Full length human recombinant protein of human BIRC7 (NP_071444) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BIRC7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
Theoretical MW
32.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.36 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Livin Antibody (OTI1D12)

  • baculoviral IAP repeat containing 7
  • baculoviral IAP repeat-containing 7
  • BIRC7
  • KIAP
  • KIAPRING finger protein 50
  • Kidney inhibitor of apoptosis protein
  • livin inhibitor-of-apoptosis
  • Livin
  • Melanoma inhibitor of apoptosis protein
  • ML-IAP
  • MLIAPlivin inhibitor of apoptosis
  • ML-IAPmliap
  • RNF50LIVINbaculoviral IAP repeat-containing protein 7

Background

The protein encoded by this gene is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Two transcript variants encoding different isoforms have been found for this gene. The two isoforms have different antiapoptotic properties, with isoform alpha protecting cells from apoptosis induced by staurosporine and isoform b protecting cells from apoptosis induced by etoposide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.
Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.
Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.
Livin: On a Prayer
Livin is a member of the inhibitor of apoptosis proteins (IAP) family that regulates programmed cell death. The Livin protein contains a single baculovirus IAP repeat (BIR) essential for function, along with a COOH-terminal RING-type zinc finger domai...  Read full blog post.
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Bioinformatics

Gene Symbol BIRC7
Entrez