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Recombinant Human LIN-28A His Protein

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SDS-Page: Lin28 Protein [NBP1-30275] - Lin28, 21.1 kDa (191aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE
SDS-Page: Lin28 Protein [NBP1-30275] - Lin28, 21.1 kDa (191aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

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Recombinant Human LIN-28A His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 42-209 of Human LIN-28A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH RSMGICKWFN VRMGFGFLSM TARAGVALDP PVDVFVHQSK LHMEGFRSLK EGEAVEFTFK KSAKGLESIR VTGPGGVFCI GSERRPKGKS MQKRRSKGDR CYNCGGLDHH AKECKLPPQP KKCHFCQSIS HMVASCPLKA QQGPSAQGKP TYFREEEEEI HSPTLLPEAQ N
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
LIN28A
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.1 M NaCl, 0.1 mM PMSF
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human LIN-28A His Protein

  • CSDD1
  • CSDD1lin-28A
  • FLJ12457
  • lin-28 homolog (C. elegans)
  • lin-28 homolog A (C. elegans)
  • LIN28
  • LIN-28
  • LIN28A
  • LIN-28A
  • LIN28RNA-binding protein LIN-28
  • Tex17
  • ZCCHC1
  • ZCCHC1protein lin-28 homolog A
  • Zinc finger CCHC domain-containing protein 1
  • zinc finger, CCHC domain containing 1

Background

Lin-28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. It is marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. It has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Recombinant Lin28 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for LIN-28A Protein (NBP1-30275) (0)

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FAQs for LIN-28A Protein (NBP1-30275). (Showing 1 - 1 of 1 FAQ).

  1. May I ask whether NBP1-30275 protein is functionally active?
    • This is a recombinant protein (His-tagged) and we have not tested its functional activity in our lab yet.

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Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.
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Bioinformatics

Gene Symbol LIN28A