![Western Blot: Lamin B2 Antibody (144) [NBP2-43834] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [144]. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-144-Western-Blot-NBP2-43834-img0007.jpg "Western Blot: Lamin B2 Antibody (144) [NBP2-43834] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [144]. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | 144 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Lamin B2. The exact sequence is proprietary. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LMNB2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
| Publication using NBP2-43834 | Applications | Species |
|---|---|---|
| Morinishi T, Tokuhara Y, Ohsaki H et al. Activation and Expression of Peroxisome Proliferator-Activated Receptor Alpha Are Associated with Tumorigenesis in Colorectal Carcinoma PPAR Res 2019-07-03 [PMID: 31354797] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Lamin B2 Antibody (NBP2-43834)Find related products by research area.
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| Gene Symbol | LMNB2 |
![Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of HeLa cells were fixed in 2% paraformaldehyde for 30 min. Red: LMNB2 protein stained by Lamin B2 antibody [144] diluted at 1:1000. Green: alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-144-Immunocytochemistry-Immunofluorescence-NBP2-43834-img0001.jpg "Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of HeLa cells were fixed in 2% paraformaldehyde for 30 min. Red: LMNB2 protein stained by Lamin B2 antibody [144] diluted at 1:1000. Green: alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]")
![Immunohistochemistry-Paraffin: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of Cal27 xenograft. Lamin B2 antibody [144] dilution: 1:200.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-144-Immunohistochemistry-Paraffin-NBP2-43834-img0002.jpg "Immunohistochemistry-Paraffin: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of Cal27 xenograft. Lamin B2 antibody [144] dilution: 1:200.")
![Western Blot: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of A. 30 ug HepG2 whole cell lysate/extract B. 30 ug HepG2 cytosol lysate/extract C. 30 ug HepG2 nuclear lysate/extract 7.5 % SDS-PAGE Lamin B2 antibody [144] dilution: 1:1000.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-144-Western-Blot-NBP2-43834-img0004.jpg "Western Blot: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of A. 30 ug HepG2 whole cell lysate/extract B. 30 ug HepG2 cytosol lysate/extract C. 30 ug HepG2 nuclear lysate/extract 7.5 % SDS-PAGE Lamin B2 antibody [144] dilution: 1:1000.")
![Western Blot: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 7.5 % SDS-PAGE Lamin B2 antibody [144] dilution: 1:1000](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-144-Western-Blot-NBP2-43834-img0005.jpg "Western Blot: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 7.5 % SDS-PAGE Lamin B2 antibody [144] dilution: 1:1000")
![Immunoprecipitation: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of Lamin B2 by 3 ug of Lamin B2 antibody [144] 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [144] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Lamin-B2-Antibody-144-Immunoprecipitation-NBP2-43834-img0003.jpg "Immunoprecipitation: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of Lamin B2 by 3 ug of Lamin B2 antibody [144] 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [144] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.")
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