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LAG-3 Antibody (1009611) [Unconjugated]

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Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human LAG-3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog # MAB23195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LAG-3 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human LAG-3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LAG-3 Monoclonal Antibody ...read more
Human peripheral blood mononuclear cells (PBMCs) were either untreated (bottom panel) or treated with with 5 µg/mL PHA (top panel) for 5 days. PBMCs were stained with Mouse Anti-Human LAG-3 Monoclonal Antibody (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
1009611
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB23195U-100UG
Alexa Fluor 405
FAB23195V-100UG
Alexa Fluor 488
FAB23195G-100UG
Alexa Fluor 532
FAB23195X
Alexa Fluor 594
FAB23195T-100UG
Alexa Fluor 647
FAB23195R-100UG
Alexa Fluor 700
FAB23195N-100UG
Alexa Fluor 750
FAB23195S-100UG
Allophycocyanin
FAB23195A
Allophycocyanin/Cy7
FAB23195APCCY7
Biotin
FAB23195B
CoraFluor 1
FAB23195CL1
DyLight 350
FAB23195D
DyLight 405
FAB23195E
DyLight 488
FAB23195K
DyLight 550
FAB23195L
DyLight 594
FAB23195M
DyLight 650
FAB23195W
DyLight 680
FAB23195Y
DyLight 755
FAB23195Z
FITC
FAB23195F
HRP
FAB23195H
Janelia Fluor 525
FAB23195JF525
Janelia Fluor 549
FAB23195I
Janelia Fluor 585
FAB23195JF585
Janelia Fluor 635
FAB23195JF635
Janelia Fluor 646
FAB23195J
Janelia Fluor 669
FAB23195JF669
PE
FAB23195P
PE/Atto594
FAB23195PEATT594
PE/Cy5.5
FAB23195PECY55
PE/Cy7
FAB23195PECY7
PerCP
FAB23195C
mFluor Violet 450 SE
FAB23195MFV450
mFluor Violet 500 SE
FAB23195MFV500
mFluor Violet 610 SE
FAB23195MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB23195-SP
Carrier Free
FAB23195U-100UG
Carrier Free
FAB23195T-100UG
Carrier Free
FAB23195S-100UG
Carrier Free
FAB23195R-100UG
Carrier Free
FAB23195N-100UG
Carrier Free
FAB23195G-100UG
Carrier Free
FAB23195V-100UG
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LAG-3 Antibody (1009611) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAG-3
Leu23-Leu450
Accession # P18627
Specificity
Detects human LAG-3 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAG-3 Antibody (1009611) [Unconjugated]

  • CD223 antigen
  • CD223
  • LAG3
  • LAG-3
  • lymphocyte activating 3
  • lymphocyte activation gene 3 protein
  • lymphocyte-activation gene 3
  • Secreted lymphocyte activation gene 3 protein
  • sLAG-3

Background

LAG-3 (Lymphocyte activation gene-3), also known as CD223, is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 and CD4 molecules share < 20% aa sequence homology but have a similar structure (1, 2). Both molecules bind to MHC class II. LAG-3 binds to MHC class II with higher affinity compared to CD4. Both LAG-3 and CD4 genes are located on the distal part of the short arm of chromosome 12.

LAG-3 is an activation-induced molecule, expressed on activated T cells and NK cells, but not on resting T cells. Studies using LAG-3 -/- mice have shown significant delay of T cell apoptosis following antigen stimulation and increased size of memory T cells pool following infection (3, 4). It also has been reported that anti-LAG-3 antibodies up-regulate T cell activation by blocking interaction of LAG-3 and MHC class II. The study has demonstrated that LAG-3 is selectively expressed on activated CD4+CD25+ TReg cells and plays a role in their suppressive activity (5). This evidence indicated, unlike the interaction of CD4 with MHC class II that plays a positive role in T cell activation, LAG-3 binds to MHC class II and negatively regulates T cell activation through LAG-3 signaling. On the other hand, studies have shown that binding of LAG-3 to MHC class II molecules on antigen presenting cells induce maturation of dendritic cells and cytokine secretion by monocytes through MHC class II signal transduction (6). Taken together, LAG-3 may have two major functions, it negatively regulates T cells activation through LAG-3 signaling and stimulates antigen presenting cells which express MHC class II.

  1. Triebel, F. et al. (1990) J. Exp. Med. 171:1393.
  2. Baixeras, E. et al. (1992) J. Exp. Med 176:327.
  3. Workman, C.J. and D.A. Vignali (2003) Eur. J. Immunol. 33:970.
  4. Workman, C.J. et al. (2004) J. Immunol. 172:5450.
  5. Huang, C.T. et al. (2004) Immunity 21:503.
  6. Andreae, S. et al. (2003) Blood 102:2130.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for LAG-3 Antibody (MAB23195). (Showing 1 - 2 of 2 FAQ).

  1. Can this LAG-3 antibody be used in ELISA? 
    • We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).
  2. I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?
    • There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.

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