![Immunoprecipitation: KNTC1 Antibody [NB100-88131] - Detection of human ROD/KNTC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROD/KNTC1 antibody NB100-88131 used for IP at 3 ug per reaction. ROD/KNTC1 was also immunoprecipitated by rabbit anti-ROD/KNTC1 antibody NB100-88130. For blotting immunoprecipitated ROD/KNTC1, NB100-88131 was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/KNTC1-Antibody-NB100-88131-img0003.jpg "Immunoprecipitation: KNTC1 Antibody [NB100-88131] - Detection of human ROD/KNTC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROD/KNTC1 antibody NB100-88131 used for IP at 3 ug per reaction. ROD/KNTC1 was also immunoprecipitated by rabbit anti-ROD/KNTC1 antibody NB100-88130. For blotting immunoprecipitated ROD/KNTC1, NB100-88131 was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
| Reactivity | HuSpecies Glossary |
| Applications | IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 2159 and 2209 of human rough deal homolog using the numbering given in entry NP_055523.1 (GeneID 9735). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KNTC1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is not recommended for Western Blot analysis. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Immunohistochemistry PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Immunohistochemistry_16650.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for KNTC1 Antibody (NB100-88131)Find related products by research area.
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| Gene Symbol | KNTC1 |
| Entrez |
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| Uniprot |
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![Immunoprecipitation: KNTC1 Antibody [NB100-88131] - Detection of human ROD/KNTC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROD/KNTC1 antibody NB100-88131 used for IP at 3 ug per reaction. ROD/KNTC1 was also immunoprecipitated by rabbit anti-ROD/KNTC1 antibody NB100-88130. For blotting immunoprecipitated ROD/KNTC1, NB100-88131 was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/KNTC1-Antibody-Immunoprecipitation-NB100-88131-img0004.jpg "Immunoprecipitation: KNTC1 Antibody [NB100-88131] - Detection of human ROD/KNTC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROD/KNTC1 antibody NB100-88131 used for IP at 3 ug per reaction. ROD/KNTC1 was also immunoprecipitated by rabbit anti-ROD/KNTC1 antibody NB100-88130. For blotting immunoprecipitated ROD/KNTC1, NB100-88131 was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")