Klotho Antibody (775340)

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Klotho, also called Klotho-alpha , is the founding member of the Klotho family within the glycosidase-1 superfamily (1, 2). Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid (1). The 1012 amino acid (aa) type I transmembrane protein contains a 32 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and an 11 aa intracellular domain. Within the ECD, human Klotho shares 87%, 90%, 90% and 86% aa identity with mouse, rat, bovine and equine Klotho, respectively. Although a truncated 549 aa isoform predicts a soluble 70 kDa form, the form found in plasma and cerebrospinal fluid is a 130 kDa form produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho (3, 4). A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes (4). Klotho is named for the Greek goddess who spins the thread of life. The phenotype of Klotho‑deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema and premature death (2). Conversely, excess Klotho extends lifespan (5). Klotho acts as a cofactor for interaction of FGF23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate‑limiting enzyme in the synthesis of 1,25(OH)₂D₃ (vitamin D)(7). Klotho‑deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D (2, 7). Both Klotho and Klotho-beta are cofactors for FGF19 binding (8). Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (9). Klotho has been reported to downregulate insulin or IGF-1 signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone (10-12).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 3 applications: Immunohistochemistry, Immunoprecipitation, Western Blot.

Showing Publications 1 - 3 of 3.

Publications using MAB5334	Applications	Species
The, E;Zhai, Y;Yao, Q;Ao, L;Fullerton, DA;Meng, X; Molecular Interaction of Soluble Klotho with FGF23 in the Pathobiology of Aortic Valve Lesions Induced by Chronic Kidney Disease International journal of biological sciences 2024-06-17 [PMID: 38993571] (Immunohistochemistry, Mouse, Transgenic Mouse)	Immunohistochemistry	Mouse, Transgenic Mouse
J Suk Kang, SS Son, JH Lee, SW Lee, AR Jeong, ES Lee, SK Cha, CH Chung, EY Lee Protective effects of klotho on palmitate-induced podocyte injury in diabetic nephropathy PLoS ONE, 2021-04-23;16(4):e0250666. 2021-04-23 [PMID: 33891667] (Western Blot, Human)	Western Blot	Human
X Zhong, S Jagarlapud, Y Weng, M Ly, JC Rouse, K McClure, T Ishino, Y Zhang, E Sousa, J Cohen, B Tzvetkova, K Cote, JJ Scarcelli, K Johnson, J Palandra, JR Apgar, S Yaddanapud, RG Villalobos, AC Opsahl, K Lam, Q Yao, W Duan, A Sievers, J Zhou, D Ferguson, A D'Antona, R Zollner, HL Zhu, R Kriz, L Lin, V Clerin Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease J. Biol. Chem., 2020-01-31;0(0):. 2020-01-31 [PMID: 32005658] (Immunoprecipitation, Rat)	Immunoprecipitation	Rat

Array MAB5334

• Klotho Antibodies
• Klotho ELISA Kit
• Klotho Lysate
• Klotho Proteins
• Klotho Proteins and Enzymes
• Klotho ELISAs