![Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (1A7 (same as 1A7G6B6)) - Azide and BSA Free [NBP2-47968] - Human Prostate Carcinoma stained with Prostate Specific Antigen Mouse Monoclonal Antibody (1A7).](http://images.novusbio.com/fullsize/Kallikrein-3-PSA-Antibody-1A7-same-as-1A7G6B6-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47968-img0001.jpg "Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (1A7 (same as 1A7G6B6)) - Azide and BSA Free [NBP2-47968] - Human Prostate Carcinoma stained with Prostate Specific Antigen Mouse Monoclonal Antibody (1A7).")
| Reactivity | Hu, FeSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | 1A7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44887). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Kallikrein 3/PSA from human sperm plasma (Uniprot: P07288) |
| Localization | Cytoplasmic |
| Specificity | Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This monoclonal antibody is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This monoclonal antibody makes an excellent pair with monoclonal antibody A67-B/E3 for PSA tests. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KLK3 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Kallikrein 3/PSA Antibody (NBP2-47968)Find related products by research area.
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| Gene Symbol | KLK3 |
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