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ITK Antibody (5G6) - BSA Free

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Western Blot: ITK Antibody (5G6) [NBP2-37578] - Analysis using ITK mouse mAb against Jurkat cell lysate.
Western Blot: ITK Antibody (5G6) [NBP2-37578] - Analysis using ITK mouse mAb against Jurkat cell lysate.
Immunocytochemistry/ Immunofluorescence: ITK Antibody (5G6) [NBP2-37578] - Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: ITK Antibody (5G6) [NBP2-37578] - Analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).
Western Blot: ITK Antibody (5G6) [NBP2-37578] - Analysis using ITK mAb against human ITK (AA: 2-120) recombinant protein. (Expected MW is 39.7 kDa)
ELISA: ITK Antibody (5G6) [NBP2-37578] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
5G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37578-0.025ml
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ITK Antibody (5G6) - BSA Free Summary

Immunogen
Purified recombinant fragment of human ITK expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITK
Purity
Unpurified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for ITK Antibody (5G6) - BSA Free

  • EC 2.7.10
  • EC 2.7.10.2
  • EMT
  • EMTMGC126257
  • homolog of mouse T-cell itk/tsk
  • IL2-inducible T-cell kinase
  • ITK
  • Kinase EMT
  • LYK
  • LYKMGC126258
  • PSCTK2
  • T-cell-specific kinase
  • Tcsk
  • tyrosine-protein kinase ITK/TSK
  • Tyrosine-protein kinase Lyk

Background

Emtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressed in T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK), a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRC homology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well the amino-terminal myristylation signal and the negative autoregulatory phosphorylation site present in all members of the Src family of PTKs (3). Additionally, the Src tyrosine kinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ITK Antibody (5G6)
- BSA Free
NBP2-37578
ITK Antibody (5G6) - BSA Free
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF

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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ITK