ISG15 Activating Enzyme/UBE1L Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48 hours before collection. The cells were lysed in modified RIPA buffer (25 mM Tris-HCl (pH 7.6), 150 mM NaCl, 1% NP-40, 1 mM EDTA, 1X Proteinase inhibitor cocktail mix, 1 mM PMSF and 1 mM Na3PO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq].

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.

Showing Publication 1 - 1 of 1.

Publication using NBL1-17502	Applications	Species
Carrillo-VAzquez DA, JardOn-Valadez E, Torres-Ruiz J et al. Conformational changes in myeloperoxidase induced by ubiquitin and NETs containing free ISG15 from systemic lupus erythematosus patients promote a pro-inflammatory cytokine response in CD4+ T cells J Transl Med 2020-11-11 [PMID: 33176801] (WB, Human)	WB	Human

Array NBL1-17502

• ISG15 Activating Enzyme/UBE1L Antibodies
• ISG15 Activating Enzyme/UBE1L Lysate
• ISG15 Activating Enzyme/UBE1L Protein