Home » ISCU » ISCU Antibodies » ISCU Antibody (OTI4F5) - Azide and BSA Free
Novus Biologicals products are now on bio-techne.com

ISCU Antibody (OTI4F5) - Azide and BSA Free

 Submit a Review

Images

 
(
Enlarge)
Western Blot: ISCU Antibody (OTI4F5) - Azide and BSA Free [NBP2-71742] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ISCU .
Western Blot: ISCU Antibody (OTI4F5) - Azide and BSA Free [NBP2-71742] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ISCU .
Immunohistochemistry: ISCU Antibody (OTI4F5) - Azide and BSA Free [NBP2-71742] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ISCU Antibody (OTI4F5) - Azide and BSA Free [NBP2-71742] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ISCU Antibody (OTI4F5) - Azide and BSA Free [NBP2-71742] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI4F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71742AF350
Alexa Fluor 405
NBP2-71742AF405
Alexa Fluor 488
NBP2-71742AF488
Alexa Fluor 532
NBP2-71742AF532
Alexa Fluor 594
NBP2-71742AF594
Alexa Fluor 647
NBP2-71742AF647
Alexa Fluor 700
NBP2-71742AF700
Alexa Fluor 750
NBP2-71742AF750
Allophycocyanin
NBP2-71742APC
Biotin
NBP2-71742B
CoraFluor 1
NBP2-71742CL1
DyLight 350
NBP2-71742UV
DyLight 405
NBP2-71742V
DyLight 488
NBP2-71742G
DyLight 550
NBP2-71742R
DyLight 594
NBP2-71742DL594
DyLight 650
NBP2-71742C
DyLight 680
NBP2-71742FR
DyLight 755
NBP2-71742IR
FITC
NBP2-71742F
HRP
NBP2-71742H
Janelia Fluor 525
NBP2-71742JF525
Janelia Fluor 549
NBP2-71742JF549
Janelia Fluor 585
NBP2-71742JF585
Janelia Fluor 635
NBP2-71742JF635
Janelia Fluor 646
NBP2-71742JF646
Janelia Fluor 669
NBP2-71742JF669
PE
NBP2-71742PE
PerCP
NBP2-71742PCP
Unconjugated
NBP2-45436
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45436
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

ISCU Antibody (OTI4F5) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human ISCU (NP_055116) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ISCU
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
15.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ISCU Antibody (OTI4F5) - Azide and BSA Free

  • 2310020H20Rik
  • HML
  • hnifU
  • iron-sulfur cluster assembly enzyme ISCU, mitochondrial
  • iron-sulfur cluster scaffold homolog (E. coli)
  • IscU iron-sulfur cluster scaffold homolog (E. coli)
  • IscU iron-sulfur cluster scaffold homolog
  • IscU
  • ISU2
  • MGC74517
  • NIFU
  • NifU-like N-terminal domain containing
  • NifU-like N-terminal domain-containing protein
  • NifU-like protein
  • NIFUN

Background

Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NGFI-B
alpha/Nur77/NR4A1 Ant
NB100-56745
Western Blot NGFI-B alpha/Nur77/NR4A1 Antibody
Unknown NGFI-B alpha/Nur77/NR4A1 Antibody
Immunohistochemistry-Paraffin NGFI-B alpha/Nur77/NR4A1 Antibody
(3 Reviews)
(30 Publications)
Species: Hu, Mu
Applications: ChIP, ChIP, ICC/IF, IHC, IHC-P, IP, WB
Sir2.1 Antibody
NB100-1923
Western Blot Sir2.1 Antibody
(1 Review)
(2 Publications)
Species: Ce
Applications: IHC, IHC-P, IP, WB
NFS1 Antibody
NBP2-37933
Immunohistochemistry-Paraffin NFS1 Antibody
Immunohistochemistry-Paraffin NFS1 Antibody
Immunohistochemistry-Paraffin NFS1 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
HO-1/HMOX1/HSP32
Antibody (HO-1-1)
NBP1-97507
Flow Cytometry HO-1/HMOX1/HSP32 Antibody (HO-1-1)
Immunohistochemistry-Paraffin HO-1/HMOX1/HSP32 Antibody (HO-1-1)
Western Blot HO-1/HMOX1/HSP32 Antibody (HO-1-1)
(1 Review)
(20 Publications)
Species: Bv, Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
S100A9 Antibody
[Unconjugated]
AF2065
Immunocytochemistry S100A9 Antibody [Unconjugated]
Immunohistochemistry S100A9 Antibody [Unconjugated]
Immunocytochemistry S100A9 Antibody [Unconjugated]
(2 Reviews)
(34 Publications)
Species: Mu
Applications: ICC, IHC, Simple Western, WB
S100A8 Antibody
[Unconjugated]
AF3059
Immunocytochemistry S100A8 Antibody [Unconjugated]
Western Blot S100A8 Antibody [Unconjugated]
Simple Western S100A8 Antibody [Unconjugated]
(2 Reviews)
(26 Publications)
Species: Mu
Applications: ICC, Simple Western, WB
TERF2IP Antibody
NB100-292
Immunohistochemistry-Paraffin TERF2IP Antibody
Immunoprecipitation TERF2IP Antibody
Western Blot TERF2IP Antibody
(22 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Rabex5 Antibody
NBP1-49938
Western Blot Rabex5 Antibody
Immunohistochemistry-Paraffin Rabex5 Antibody
Western Blot Rabex5 Antibody
(2 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
Rap1A/B Antibody
[Unconjugated]
AF3767
Western Blot Rap1A/B Antibody [Unconjugated]
Western Blot Rap1A/B Antibody [Unconjugated]
Immunohistochemistry Rap1A/B Antibody [Unconjugated]
(4 Publications)
Species: Hu, Mu, Rt
Applications: IHC, WB
Frataxin Antibody
(OTI1C12)
NBP2-01743
Flow Cytometry Frataxin Antibody (OTI1C12)
Western Blot Frataxin Antibody (OTI1C12)
Immunohistochemistry-Paraffin Frataxin Antibody (OTI1C12)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
SLURP1 Antibody
AF4479
Immunohistochemistry SLURP1 Antibody
(1 Publication)
Species: Hu
Applications: IHC
HIST1H4F
Overexpression Lysate
NBP2-07996
Western Blot HIST1H4F Overexpression Lysate
Species: Hu
Applications: WB
H4/n Antibody
(3E7H2) - BSA Free
NBP2-52486
ELISA H4/n Antibody (3E7H2) - BSA Free
Western Blot H4/n Antibody (3E7H2) - BSA Free
Flow Cytometry H4/n Antibody (3E7H2) - BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Recombinant Human
HIST1H4I GST (N-Term) ...
H00008294-P01
SDS-PAGE Recombinant Human HIST1H4I GST (N-Term) Protein
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
H4/e
Overexpression Lysate
NBL1-11570
Western Blot H4/e Overexpression Lysate
Species: Hu
Applications: WB
H4/h Antibody (6D4)
H00008365-M01
Sandwich ELISA H4/h Antibody (6D4)
Immunocytochemistry/ Immunofluorescence H4/h Antibody (6D4)
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA
H4/j
Overexpression Lysate
NBL1-11567
Western Blot H4/j Overexpression Lysate
Species: Hu
Applications: WB
Histone H4 Antibody
AF5215
Western Blot Histone H4 Antibody
Immunocytochemistry Histone H4 Antibody
(6 Publications)
Species: Hu, Mu
Applications: ICC, WB

Publications for ISCU Antibody (NBP2-71742) (0)

There are no publications for ISCU Antibody (NBP2-71742).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ISCU Antibody (NBP2-71742) (0)

There are no reviews for ISCU Antibody (NBP2-71742). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ISCU Antibody (NBP2-71742) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ISCU Products

Blogs on ISCU

There are no specific blogs for ISCU, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.
Mouse IgG1 Isotype Control (MG1)
NBP1-97005-0.5mg

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ISCU Antibody (OTI4F5) - Azide and BSA Free and receive a gift card or discount.


Bioinformatics

Gene Symbol ISCU