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IRF9 Antibody (PCRP-IRF9-2F8)

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Immunocytochemistry/Immunofluorescence: IRF9 Antibody (PCRP-IRF9-2F8) [NBP3-13835] - Immunofluorescent analysis of PFA-fixed HeLa cells. IRF9 Antibody (PCRP-IRF9-2F8) followed by goat anti-mouse IgG-CF488 (green); counterstain (red).
Immunocytochemistry/ Immunofluorescence: IRF9 Antibody (PCRP-IRF9-2F8) [NBP3-13835] - Immunofluorescent analysis of PFA-fixed HeLa cells. IRF9 Antibody (PCRP-IRF9-2F8) followed by goat anti-mouse IgG-CF488 (green); ...read more
Flow Cytometry: IRF9 Antibody (PCRP-IRF9-2F8) [NBP3-13835] - Flow cytometric analysis of PFA-fixed HeLa cells. IRF9 antibody (PCRP-IRF9-2F8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: IRF9 Antibody (PCRP-IRF9-2F8) [NBP3-13835] - Analysis of Protein Array containing more than 19,000 full-length human proteins using IRF9 Mouse Monoclonal Antibody (PCRP-IRF9-2F8).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, MA
Clone
PCRP-IRF9-2F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-14228AF350
Alexa Fluor 405
NBP3-14228AF405
Alexa Fluor 488
NBP3-14228AF488
Alexa Fluor 532
NBP3-14228AF532
Alexa Fluor 594
NBP3-14228AF594
Alexa Fluor 647
NBP3-14228AF647
Alexa Fluor 700
NBP3-14228AF700
Alexa Fluor 750
NBP3-14228AF750
Allophycocyanin
NBP3-14228APC
Biotin
NBP3-14228B
CoraFluor 1
NBP3-14228CL1
DyLight 350
NBP3-14228UV
DyLight 405
NBP3-14228V
DyLight 488
NBP3-14228G
DyLight 550
NBP3-14228R
DyLight 594
NBP3-14228DL594
DyLight 650
NBP3-14228C
DyLight 680
NBP3-14228FR
DyLight 755
NBP3-14228IR
FITC
NBP3-14228F
HRP
NBP3-14228H
Janelia Fluor 525
NBP3-14228JF525
Janelia Fluor 549
NBP3-14228JF549
Janelia Fluor 646
NBP3-14228JF646
Janelia Fluor 669
NBP3-14228JF669
PE
NBP3-14228PE
PerCP
NBP3-14228PCP
mFluor Violet 450 SE
NBP3-14228MFV450
mFluor Violet 500 SE
NBP3-14228MFV500
mFluor Violet 610 SE
NBP3-14228MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-14228
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IRF9 Antibody (PCRP-IRF9-2F8) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14228)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant full-length human IRF9 protein (Uniprot: Q00978)
Localization
Cytoplasm, Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IRF9
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
Theoretical MW
43.34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for IRF9 Antibody (PCRP-IRF9-2F8)

  • IFN-alpha-responsive transcription factor subunit
  • interferon regulatory factor 9
  • Interferon-stimulated gene factor 3 gamma
  • interferon-stimulated transcription factor 3, gamma (48kD)
  • interferon-stimulated transcription factor 3, gamma 48kDa
  • IRF9
  • IRF-9
  • ISGF3 p48 subunit
  • ISGF3
  • ISGF3G
  • ISGF3GISGF-3 gamma
  • p48
  • Transcriptional regulator ISGF3 subunit gamma

Background

Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol IRF9
Uniprot