I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

Name: Integrin beta 1/CD29 Antibody (265917) [Unconjugated]
Brand: Novus Biologicals
SKU: MAB2405
Price: 459 USD

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Accepted Answer

I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	MuSpecies Glossary
Applications	WB, Flow, IHC, CyTOF-ready, ICC/IF
Clone	265917
Clonality	Monoclonal
Host	Rat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 FAB2405U-100UG

Alexa Fluor 405 FAB2405V-100UG

Alexa Fluor 488 FAB2405G-100UG

Alexa Fluor 532 FAB2405X

Alexa Fluor 594 FAB2405T-100UG

Alexa Fluor 647 FAB2405R-100UG

Alexa Fluor 700 FAB2405N-100UG

Alexa Fluor 750 FAB2405S-100UG

Allophycocyanin/Cy7 FAB2405APCCY7

Biotin FAB2405B

CoraFluor 1 FAB2405CL1

DyLight 350 FAB2405D

DyLight 405 FAB2405E

DyLight 488 FAB2405K

DyLight 550 FAB2405L

DyLight 594 FAB2405M

DyLight 650 FAB2405W

DyLight 680 FAB2405Y

DyLight 755 FAB2405Z

HRP FAB2405H

Janelia Fluor 525 FAB2405JF525

Janelia Fluor 549 FAB2405I

Janelia Fluor 646 FAB2405J

Janelia Fluor 669 FAB2405JF669

PE/Atto594 FAB2405PEATT594

PE/Cy5.5 FAB2405PECY55

PE/Cy7 FAB2405PECY7

Phycoerythrin FAB2405P-100

Phycoerythrin FAB2405P-025

mFluor Violet 450 SE FAB2405MFV450

mFluor Violet 500 SE FAB2405MFV500

mFluor Violet 610 SE FAB2405MFV610

Catalog# & Formulation	Size	Price
Carrier Free MAB2405-SP

Carrier Free FAB2405P-100

Carrier Free FAB2405P-025

Carrier Free FAB2405V-100UG

Carrier Free FAB2405U-100UG

Carrier Free FAB2405T-100UG

Carrier Free FAB2405S-100UG

Carrier Free FAB2405R-100UG

Carrier Free FAB2405N-100UG

Carrier Free FAB2405G-100UG

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2405U-100UG
Alexa Fluor 405	FAB2405V-100UG
Alexa Fluor 488	FAB2405G-100UG
Alexa Fluor 532	FAB2405X
Alexa Fluor 594	FAB2405T-100UG
Alexa Fluor 647	FAB2405R-100UG
Alexa Fluor 700	FAB2405N-100UG
Alexa Fluor 750	FAB2405S-100UG
Allophycocyanin/Cy7	FAB2405APCCY7
Biotin	FAB2405B
CoraFluor 1	FAB2405CL1
DyLight 350	FAB2405D
DyLight 405	FAB2405E
DyLight 488	FAB2405K
DyLight 550	FAB2405L
DyLight 594	FAB2405M
DyLight 650	FAB2405W
DyLight 680	FAB2405Y
DyLight 755	FAB2405Z
HRP	FAB2405H
Janelia Fluor 525	FAB2405JF525
Janelia Fluor 549	FAB2405I
Janelia Fluor 646	FAB2405J
Janelia Fluor 669	FAB2405JF669
PE/Atto594	FAB2405PEATT594
PE/Cy5.5	FAB2405PECY55
PE/Cy7	FAB2405PECY7
Phycoerythrin	FAB2405P-100
Phycoerythrin	FAB2405P-025
mFluor Violet 450 SE	FAB2405MFV450
mFluor Violet 500 SE	FAB2405MFV500
mFluor Violet 610 SE	FAB2405MFV610

Integrin beta 1/CD29 Antibody (265917) [Unconjugated]

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Publications for Integrin beta 1/CD29 Antibody (MAB2405)(11)

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Immunogen	Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1/CD29 Gln21-Asp728 Accession # P09055
Specificity	Detects mouse Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 3, rmIntegrin beta 4, or rmIntegrin beta 6 is observed.
Source	N/A
Isotype	IgG2a
Clonality	Monoclonal
Host	Rat
Gene	ITGB1
Purity Statement	Protein A or G purified from hybridoma culture supernatant
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS.

Publications using MAB2405	Applications	Species
R Ma, D Gong, H You, C Xu, Y Lu, G Bergers, Z Werb, OD Klein, CK Petritsch, P Lu LGL1 binds to Integrin beta1 and inhibits downstream signaling to promote epithelial branching in the mammary gland Cell Reports, 2022-02-15;38(7):110375. 2022-02-15 [PMID: 35172155] (IHC, Mouse)	IHC	Mouse
M Bieri, R Hendrickx, M Bauer, B Yu, T Jetzer, B Dreier, PRE Mittl, J Sobek, A Plückthun, UF Greber, S Hemmi The RGD-binding integrins alphavbeta6 and alphavbeta8 are receptors for mouse adenovirus-1 and -3 infection PloS Pathogens, 2021-12-15;17(12):e1010083. 2021-12-15 [PMID: 34910784] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
A Adolf, P Turko, A Rohrbeck, I Just, I Vida, G Ahnert-Hil, M Höltje The Higher Sensitivity of GABAergic Compared to Glutamatergic Neurons to Growth-Promoting C3bot Treatment Is Mediated by Vimentin Front Cell Neurosci, 2020-11-03;14(0):596072. 2020-11-03 [PMID: 33240046] (ICC, Rat)	ICC	Rat
M Reed, AC Luissint, V Azcutia, S Fan, MN O'Leary, M Quiros, J Brazil, A Nusrat, CA Parkos Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo Nat Commun, 2019-11-01;10(1):5004. 2019-11-01 [PMID: 31676794] (IF, Mouse)	IF	Mouse
T Iwanaga, J Nio-Kobaya, H Takahashi- Bush-like integrin filament networks associated with hyaloid vasculature in murine neonate eyes Biomed. Res., 2019-01-01;40(2):79-85. 2019-01-01 [PMID: 30982803] (IHC, Mouse)	IHC	Mouse
A Gorecka, S Salemi, D Haralampie, F Moalli, D Stroka, D Candinas, D Eberli, L Brügger Autologous transplantation of adipose-derived stem cells improves functional recovery of skeletal muscle without direct participation in new myofiber formation Stem Cell Res Ther, 2018-07-17;9(1):195. 2018-07-17 [PMID: 30016973] (Flow Cytometry, ICC, Mouse)	Flow Cytometry, ICC	Mouse
A Kishimoto, S Kimura, J Nio-Kobaya, H Takahashi-, AM Park, T Iwanaga Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy Exp. Eye Res., 2018-03-27;172(0):1-9. 2018-03-27 [PMID: 29596849] (IHC, Mouse)	IHC	Mouse
Cyclooxygenase-2 induced ?1-integrin expression in NSCLC and promoted cell invasion via the EP1/MAPK/E2F-1/FoxC2 signal pathway Sci Rep, 2016-09-22;6(0):33823. 9/22/2016 [PMID: 27654511]
Antibody-based targeting of alternatively spliced tissue factor: a new approach to impede the primary growth and spread of pancreatic ductal adenocarcinoma Oncotarget, 2016-05-03;7(18):25264-75. 2016-05-03 [PMID: 26967388] (IHC-P, Neutralization, Mouse)	IHC-P, Neutralization	Mouse
Richard JF, Roy M, Audoy-Remus J, Tremblay P, Vallieres L Crawling Phagocytes Recruited in the Brain Vasculature after Pertussis Toxin Exposure through IL6, ICAM1 and ITGalphaM. Brain Pathol., 2011-05-23;21(0):661. 2011-05-23 [PMID: 21418369] (Neutralization, Mouse)	Neutralization	Mouse
Habtezion A, Toivola DM, Asghar MN, Kronmal GS, Brooks JD, Butcher EC, Omary MB Absence of keratin 8 confers a paradoxical microflora-dependent resistance to apoptosis in the colon. Proc. Natl. Acad. Sci. U.S.A., 2011-01-10;108(4):1445-50. 2011-01-10 [PMID: 21220329] (IHC-Fr, Western Blot, Mouse)	IHC-Fr, Western Blot	Mouse
Show All 11 Publications. Collapse Publications.

Gene Symbol	ITGB1
Uniprot	P09055()