![Flow Cytometry: Integrin alpha D/CD11d Antibody (CA11.8H2) [NB100-65292] - Staining of canine peripheral blood lymphocytes with Mouse anti Dog CD11d:Alexa Fluor® 647](http://images.novusbio.com/fullsize/Integrin-alpha-D-CD11d-Antibody-CA11-8H2-Flow-Cytometry-NB100-65292-img0001.jpg "Flow Cytometry: Integrin alpha D/CD11d Antibody (CA11.8H2) [NB100-65292] - Staining of canine peripheral blood lymphocytes with Mouse anti Dog CD11d:Alexa Fluor® 647")
| Reactivity | CaSpecies Glossary |
| Applications | Flow, IHC, IP |
| Clone | CA11.8H2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | Affinity purified CD11 antigen from canine spleen |
| Specificity | NB100-65292 recognizes the canine CD11d cell surface antigen (the integrin ad sub-unit). The ad integrin is a newly recognized integrin chain that is expressed as a heterodimer with the b2 integrin (CD18). CD11d is expressed by a sub-population of CD8 positive T cells, macrophages and by gd T cells in the splenic red pulp. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ITGAD |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
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| Publication using NB100-65292 | Applications | Species |
|---|---|---|
| Bennett AL, Williams LE, Ferguson MW et al. Canine acute leukaemia: 50 cases (1989-2014) Vet Comp Oncol 2016-07-12 [PMID: 27402031] (FLOW, Canine) | FLOW | Canine |
Secondary Antibodies |
Isotype Controls |
Research Areas for Integrin alpha D/CD11d Antibody (NB100-65292)Find related products by research area.
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| Gene Symbol | ITGAD |
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")