Integrin alpha 4/CD49d Antibody (PS/2) [FITC]

Protect conjugated forms from light.

VLA-4 belongs to the beta1-integrin family, which functions both as cell-matrix and cell-cell receptors. (1-3) As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. (1, 2) In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. (2, 3) CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive. (1, 2) Antibody PS/2 also reacts with human VLA-4. (1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	Flow	Mouse	04/04/2019	View Summary Application Flow Cytometry Sample Tested Lymph node cells Species Mouse Lot A5110-Y6000G Comments Comments Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and periaortic and inguinal lymph node cells were isolated when the animals were at the onset of symptoms. Cells were stained for VLA4, CD4, CD45, LFA-1, TCRb, CD28, CTLA-4, CD40L, OX40, PD1 and CD11b, plus for viability to exclude dead cells. VLA4 staining is showed in CD4+ cells from a control sample not stained for VLA4 (red) and an experimental sample stained for VLA.

Array NBP1-26662

• Integrin alpha 4/CD49d Antibodies
• Integrin alpha 4/CD49d ELISA Kit
• Integrin alpha 4/CD49d Lysate
• Integrin alpha 4/CD49d Proteins