Rat iNOS ELISA Kit (Colorimetric)

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA, In-vitro.

Showing Publications 1 - 5 of 5.

Publications using NBP2-80257	Applications	Species
Sayed DF, Mohamed MA, Nada AS et al. Hepatoprotective role of myricitrin isolated from Mimusops elengi Linn. leaves extract on ?-radiation-induced liver damage in rats: Phyto-biochemical investigations Cell biochemistry and function 2023-06-21 [PMID: 37342005] (ELISA)	ELISA
Abdel-Rafei MK, Thabet NM, Amin MM Concerted regulation of OPG/RANKL/ NF kappa B/MMP-13 trajectories contribute to ameliorative capability of prodigiosin and/or low dose gamma-radiation against adjuvant- induced arthritis in rats International immunopharmacology 2022-08-06 [PMID: 35944459] (In-vitro, Human)	In-vitro	Human
Mohamed B, Ismail R, Saleh I et al. Olmesartan Ameliorates Cyclophosphamide-induced Hemorrhagic Cystitis in Rats via Nrf2/HO-1 Signaling Pathway Tissue and Cell 2022-07-01 [PMID: 35930992] (ELISA) Details: Adult male Wistar rats	ELISA
Hussein RM, Kandeil MA, Mohammed NA, Khallaf RA Evaluation of the hepatoprotective effect of curcumin-loaded solid lipid nanoparticles against paracetamol overdose toxicity: Role of inducible nitric oxide synthase Journal of liposome research 2022-02-08 [PMID: 35132919] (ELISA)	ELISA
Zhang Y, Zhang X, Cao Z Et al. Endothelial cell-derived SSAO can increase MLC20 phosphorylation in VSMCs Open Life Sciences 2021-10-21 [PMID: 34722886] (ELISA)	ELISA

Array NBP2-80257

• iNOS Antibodies
• iNOS ELISA Kits
• iNOS Lysate
• iNOS Proteins