Reactivity | MuSpecies Glossary |
Applications | WB, ELISA(Det) |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Biotin |
Concentration | LYOPH |
Immunogen | E. coli-derived recombinant mouse IL-11 (R&D Systems, Catalog # 418-ML) Gly23-Leu199 Accession # P47873 |
Specificity | Detects mouse IL-11 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human (rh) IL-11, rmCT-1, rrCNTF, rmG-CSF, rmIL-6, rmLIF, and rmOSM is observed. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | IL11 |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | ELISA Capture: Mouse IL-11 Antibody (Catalog # MAB4181) ELISA Detection: Mouse IL-11 Biotinylated Antibody (Catalog # BAF418) Standard: Recombinant Mouse IL-11 (Catalog # 418-ML) |
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Readout System | ||
Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Interleukin-11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1 alpha -stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T1165. IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF). The mouse IL-11 cDNA encodes a 199 amino acid residue precursor polypeptide with a 22 amino acid residue hydrophobic signal that is processed proteolytically to generate the 177 amino acid residue mature protein. IL-11 contains no cysteine residues or potential glycosylation sites.
IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cells. IL-11, in the presence of IL-3 or SCF, has also been shown to stimulate erythropoiesis. Among nonhematopoietic cell populations, IL-11, like IL-6 and LIF, can stimulate the synthesis of hepatic acute-phase proteins. Consistent with the in vitro functions of IL‑11, in vivo administration of rhIL-11 in normal mice was found to enhance the generation of Ig producing cells and platelets, and to increase the cycling rates of bone marrow-derived CFU-GM, BFU-E, and CFU-GEMM progenitors. IL-11 exerts its biological activities through binding to a specific high-affinity receptor complex consisting of an IL-11 receptor alpha chain and gp130.
Secondary Antibodies |
Isotype Controls |
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