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Donkey anti-Goat IgG Secondary Antibody [HRP Polymer] (IHC-Prediluted)

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HAI-1 was detected in paraffin-embedded sections of human lung cancer using Goat Anti-Human HAI‑1 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) at 1 μg/mL for 1 hour at room temperature ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP Polymer
Format
IHC-Prediluted

Donkey anti-Goat IgG Secondary Antibody [HRP Polymer] (IHC-Prediluted) Summary

Immunogen
Goat IgG
Specificity
Detects heavy ( gamma ) chains on goat IgG and light chains on all goat immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin goat serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Immunohistochemistry
Publications
Read Publications using
VC004 in the following applications:

Packaging, Storage & Formulations

Storage
6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied as a solution in PBS with preservative.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Goat IgG Secondary Antibody [HRP Polymer] (IHC-Prediluted)

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (VC004)(8)

We have publications tested in 5 confirmed species: Human, Mouse, Goat, Hamster, Transgenic Mouse.

We have publications tested in 2 applications: IHC, IHC-P.


Filter By Application
IHC
(6)
IHC-P
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
Goat
(1)
Hamster
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using VC004 Applications Species
L Wiedmann, F De Angelis, N Vaquero-Si, E Donato, E Espinet, I Moll, E Alsina-San, H Bohnenberg, E Fernandez-, R Mülfarth, M Vacca, J Gerwing, LC Conradi, P Ströbel, A Trumpp, C Mogler, A Fischer, J Rodriguez- HAPLN1 potentiates peritoneal metastasis in pancreatic cancer Nature Communications, 2023-04-24;14(1):2353. 2023-04-24 [PMID: 37095087] (IHC, Goat) IHC Goat
X Liu, HS Park, Y Matsuoka, C Santos, L Yang, C Luongo, IN Moore, RF Johnson, NL Garza, P Zhang, P Lusso, SM Best, UJ Buchholz, CL Nouën Live-attenuated pediatric parainfluenza vaccine expressing 6P-stabilized SARS-CoV-2 spike protein is protective against SARS-CoV-2 variants in hamsters bioRxiv : the preprint server for biology, 2022-12-13;0(0):. 2022-12-13 [PMID: 36561185] (IHC, Hamster) IHC Hamster
JR Sheldon, LE Himmel, DE Kunkle, AJ Monteith, KN Maloney, EP Skaar Lipocalin-2 is an essential component of the innate immune response to Acinetobacter baumannii infection PloS Pathogens, 2022-09-02;18(9):e1010809. 2022-09-02 [PMID: 36054235] (IHC, Mouse) IHC Mouse
Y Yamada, Y Zhong, S Miki, A Taura, TH Rabbitts The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis Scientific Reports, 2022-05-04;12(1):7226. 2022-05-04 [PMID: 35508511] (IHC, Transgenic Mouse) IHC Transgenic Mouse
L Vitale-Cro, I Szalayova, A Scoggins, M Palkovits, E Mezey SARS-CoV-2 entry sites are present in all structural elements of the human glossopharyngeal and vagal nerves: Clinical implications EBioMedicine, 2022-04-04;78(0):103981. 2022-04-04 [PMID: 35390636] (IHC, Human) IHC Human
C Nadolny, X Zhang, Q Chen, SF Hashmi, W Ali, C Hemme, N Ahsan, Y Chen, R Deng Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma American journal of cancer research, 2021-10-15;11(10):4746-4767. 2021-10-15 [PMID: 34765291] (IHC, Mouse) IHC Mouse
M Miki, T Oono, N Fujimori, T Takaoka, K Kawabe, Y Miyasaka, T Ohtsuka, D Saito, M Nakamura, Y Ohkawa, Y Oda, M Suyama, T Ito, Y Ogawa CLEC3A, MMP7, and LCN2 as novel markers for predicting recurrence in resected G1 and G2 pancreatic neuroendocrine tumors Cancer Med, 2019-05-25;0(0):. 2019-05-25 [PMID: 31129920] (Human) Human
H Hu, Z Liu, C Liu Correlation of OPN gene expression with proliferation and apoptosis of ovarian cancer cells and prognosis of patients Oncol Lett, 2019-01-07;17(3):2788-2794. 2019-01-07 [PMID: 30854053] (IHC-P, Human) IHC-P Human

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Understanding the Importance of Human IgG
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