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Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

Images

 
Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9B Polyclonal Antibody (Catalog # AF3584) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This ...read more
Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog #AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP (Horseradish Peroxidase)

Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary

Immunogen
Goat IgG
Specificity
Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Simple Western
  • Western Blot
Publications
Read Publications using
HAF109 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied as a 0.2 um filtered solution in PBS with stabilizers.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (HAF109)(127)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Bacteria, Bovine, Canine, Goat, N/A, Virus.

We have publications tested in 11 applications: Bioassay, Co-Immunoprecipitation, ELISA Detection, ELISA Development, Flow Cytometry, IHC, IHC-Fr, Secondary Antibody, Simple Western, Western Blot, Western Blots.


Filter By Application
Bioassay
(1)
Co-Immunoprecipitation
(1)
ELISA Detection
(2)
ELISA Development
(2)
Flow Cytometry
(1)
IHC
(1)
IHC-Fr
(1)
Secondary Antibody
(1)
Simple Western
(1)
Western Blot
(42)
Western Blots
(1)
All Applications
Filter By Species
Human
(29)
Mouse
(17)
Rat
(3)
Bacteria
(1)
Bovine
(1)
Canine
(1)
Goat
(1)
N/A
(1)
Virus
(1)
All Species
Showing Publications 1 - 10 of 127. Show All 127 Publications.
Publications using HAF109 Applications Species
Bhattacharya, R;Ward, T;Kalejaiye, TD;Mishra, A;Leeman, S;Arzaghi, H;Seidman, JG;Seidman, CE;Musah, S; Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations bioRxiv : the preprint server for biology 2024-08-06 [PMID: 39211233] (Western Blot, Human) Western Blot Human
Fujii, K;Fujishima, Y;Kita, S;Kawada, K;Fukuoka, K;Sakaue, TA;Okita, T;Kawada-Horitani, E;Nagao, H;Fukuda, S;Maeda, N;Nishizawa, H;Shimomura, I; Pharmacological HIF-1 activation upregulates extracellular vesicle production synergistically with adiponectin through transcriptional induction and protein stabilization of T-cadherin Scientific reports 2024-02-13 [PMID: 38351156] (Western Blot, Mouse) Western Blot Mouse
Martin Flores, N;Podpolny, M;McLeod, F;Workman, I;Crawford, K;Ivanov, D;Leonenko, G;Escott-Price, V;Salinas, PC; Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer's disease, restores synapse integrity and memory in a disease mouse model eLife 2024-01-29 [PMID: 38285009] (Western Blot, Human) Western Blot Human
Rybakova, EY;Avdonin, PP;Trufanov, SK;Goncharov, NV;Avdonin, PV; Synergistic Interaction of 5-HT1B and 5-HT2B Receptors in Cytoplasmic Ca2+ Regulation in Human Umbilical Vein Endothelial Cells: Possible Involvement in Pathologies International journal of molecular sciences 2023-09-08 [PMID: 37762136] (Western Blot, Human) Western Blot Human
Shrestha, M;Wang, DY;Ben-David, Y;Zacksenhaus, E; CDK4/6 inhibitors and the pRB-E2F1 axis suppress PVR and PD-L1 expression in triple-negative breast cancer Oncogenesis 2023-05-26 [PMID: 37230983] (Western Blot, Mouse) Western Blot Mouse
L Wiedmann, F De Angelis, N Vaquero-Si, E Donato, E Espinet, I Moll, E Alsina-San, H Bohnenberg, E Fernandez-, R Mülfarth, M Vacca, J Gerwing, LC Conradi, P Ströbel, A Trumpp, C Mogler, A Fischer, J Rodriguez- HAPLN1 potentiates peritoneal metastasis in pancreatic cancer Nature Communications, 2023-04-24;14(1):2353. 2023-04-24 [PMID: 37095087] (Western Blot, Goat) Western Blot Goat
Alvin Phan, Hugo Avila, J. Andrew MacKay Biomimetic SARS-CoV-2 Spike Protein Nanoparticles Biomacromolecules 5/8/2023 [PMID: 37001147]
KE Stephenson, J Porte, A Kelly, WA Wallace, CE Huntington, CL Overed-Say, ES Cohen, RG Jenkins, AE John The IL-33:ST2 axis is unlikely to play a central fibrogenic role in idiopathic pulmonary fibrosis Respiratory Research, 2023-03-23;24(1):89. 3/23/2023 [PMID: 36949463]
Pauline P. Marie, Shih‐Jung Fan, John Mason, Adam Wells, Cláudia C. Mendes, S. Mark Wainwright et al. Accessory ESCRT‐III proteins are conserved and selective regulators of Rab11a‐exosome formation Journal of Extracellular Vesicles 3/5/2023 [PMID: 36872252]
Hiroyuki Suzuki, Hideki Iwamoto, Takahiro Seki, Toru Nakamura, Atsutaka Masuda, Takahiko Sakaue et al. Tumor‐derived insulin‐like growth factor‐binding protein‐1 contributes to resistance of hepatocellular carcinoma to tyrosine kinase inhibitors Cancer Communications 4/1/2023 [PMID: 36825684]
Show All 127 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IgG Antibody (HAF109) (0)

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Bioinformatics

Gene Symbol IGHG1