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Donkey anti-Mouse IgG Secondary Antibody [NL493]

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Detection of human Vimentin by incubating cells with 8 μg/mL of Mouse anti-Human Vimentin monoclonal antibody (MAB21052) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Mouse ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, ICC/IF
Clonality
Polyclonal
Host
Donkey
Conjugate
NL493

Order Details

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View Available Formulations
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Donkey anti-Mouse IgG Secondary Antibody [NL493] Summary

Additional Information
NorthernLights 493 Fluorochrome-labeled Antibody, X Absorbed
Immunogen
Mouse IgG
Specificity
Mouse IgG (H+L)
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
Publications
Read Publications using
NL009 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Mouse IgG Secondary Antibody [NL493]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (NL009)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC, IHC, IHC-P, Immunohistochemistry.


Filter By Application
ICC
(5)
IHC
(4)
IHC-P
(1)
Immunohistochemistry
(1)
All Applications
Filter By Species
Human
(7)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NL009 Applications Species
Hussain, SS;Libby, EF;Lever, JEP;Tipper, JL;Phillips, SE;Mazur, M;Li, Q;Campos-Gómez, J;Harrod, KS;Rowe, SM; ACE-2 Blockade &amp; TMPRSS2 Inhibition Mitigate SARS-CoV-2 Severity Following Cigarette Smoke Exposure in Airway Epithelial Cells In Vitro bioRxiv : the preprint server for biology 2024-06-24 [PMID: 38979208] (Immunohistochemistry, Human) Immunohistochemistry Human
GAE Pickering, F Felix-Ilem, MJ Clark, K Kocsy, J Simpson, I Bellantuon, A Gartland, JM Wilkinson, K Hatzikotou, E Kiss-Toth The Kinesin Gene KIF26B Modulates the Severity of Post-Traumatic Heterotopic Ossification International Journal of Molecular Sciences, 2022-08-16;23(16):. 2022-08-16 [PMID: 36012474] (ICC, Human) ICC Human
K Chakrabart, KN Prashanthi, R Shetty, S Argulwar, N Jeyabalan, A Ghosh Generation of iPSC line (GLNNFi001-A) from peripheral blood mononuclear cells of a patient with macular corneal dystrophy Stem Cell Research, 2022-04-22;62(0):102789. 2022-04-22 [PMID: 35472829] (ICC, Human) ICC Human
Ameneh Mehri Ghahfarrokhi, Mohammad Saeid Jami, Morteza Hashemzadeh Chaleshtori Upregulation of Neuroprogenitor and Neural Markers via Enforced miR-124 and Growth Factor Treatment Int J Mol Cell Med 2020-08-25 [PMID: 32832485]
G Kaufman, NM Kiburi, D Skrtic The self-renewal dental pulp stem cell microtissues challenged by a toxic dental monomer Biosci. Rep., 2020-06-26;40(6):. 2020-06-26 [PMID: 32495822] (ICC, Human) ICC Human
C Tashita, M Hoshi, A Hirata, K Nakamoto, T Ando, T Hattori, Y Yamamoto, H Tezuka, H Tomita, A Hara, K Saito Kynurenine plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate-induced colitis in mice World J. Gastroenterol., 2020-03-07;26(9):918-932. 2020-03-07 [PMID: 32206003] (IHC, Mouse) IHC Mouse
M Zein-Hammo, PR Standley Optimized Modeled Myofascial Release Enhances Wound Healing in 3-Dimensional Bioengineered Tendons: Key Roles for Fibroblast Proliferation and Collagen Remodeling J Manipulative Physiol Ther, 2019-11-23;0(0):. 2019-11-23 [PMID: 31771832] (IHC, Human) IHC Human
Enhancing the Three-Dimensional Structure of Adherent Gingival Fibroblasts and Spheroids via a Fibrous Protein-Based Hydrogel Cover Cells Tissues Organs (Print), 2016-08-30;0(0):. 2016-08-30 [PMID: 27578009] (IHC, Mouse) IHC Mouse
Junfeng Zhang, Hongying Tan, Wei Jiang, Zhiyi Zuo Amantadine Alleviates Postoperative Cognitive Dysfunction Possibly by Increasing Glial Cell Line-derived Neurotrophic Factor in Rats Anesthesiology 2014-10-01 [PMID: 25251457]
Wang Z, Huang W, Zuo Z Perioperative aspirin improves neurological outcome after focal brain ischemia possibly via inhibition of Notch 1 in rat. J Neuroinflammation, 2014-03-25;11(0):56. 2014-03-25 [PMID: 24661619] (IHC-P, Rat) IHC-P Rat
Show All 14 Publications.

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Product General Protocols

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FAQs for IgG Antibody (NL009) (0)

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Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHG1