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Donkey anti-Mouse IgG Secondary Antibody [NL557]

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Detection of human Vimentin by incubating cells with 8 μg/mL of Mouse anti-Human Vimentin monoclonal antibody (MAB21052) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Mouse ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, ICC/IF
Clonality
Polyclonal
Host
Donkey
Conjugate
NL557
Suitable Sample Type
23042

Order Details

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View Available Formulations
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Donkey anti-Mouse IgG Secondary Antibody [NL557] Summary

Additional Information
NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed
Immunogen
Mouse IgG
Specificity
Mouse IgG (H+L)
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
Publications
Read Publications using
NL007 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Mouse IgG Secondary Antibody [NL557]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (NL007)(30)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 6 applications: Cell Culture, ICC, IHC, IHC-Fr, IHC-P, Immunocytochemistry.


Filter By Application
Cell Culture
(1)
ICC
(13)
IHC
(3)
IHC-Fr
(2)
IHC-P
(2)
Immunocytochemistry
(1)
All Applications
Filter By Species
Human
(16)
Mouse
(4)
Rat
(1)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NL007 Applications Species
Tonin, R;Feo, F;Falliano, S;Giunti, L;Calamai, M;Procopio, E;Mari, F;Sciruicchio, V;Conti, V;Fanelli, I;Bambi, F;Guerrini, R;Morrone, A; Generation of a human induced pluripotent stem cell line from a patient with GM3 synthase deficiency using self-replicating RNA vector Stem cell research 2024-05-03 [PMID: 38703669] (Immunocytochemistry, Human) Immunocytochemistry Human
AA Hill, M Kim, DF Zegarra-Ru, LC Chang, KC Norwood, A Assié, WH Wu, MC Renfroe, HW Song, AM Major, BS Samuel, JM Hyser, RS Longman, GE Diehl Acute high fat diet impairs macrophage supported intestinal damage resolution JCI Insight, 2023-02-08;0(0):. 2023-02-08 [PMID: 36538527] (IHC, Mouse) IHC Mouse
Maya Rozenfeld, Ivana Savic Azoulay, Tsipi Ben Kasus Ben Kasus Nissim, Alexandra Stavsky, Moran Melamed, Grace Stutzmann et al. Essential role of the mitochondrial Na+/Ca2+ exchanger NCLX in mediating PDE2-dependent neuronal survival and learning Cell Reports 2022-12-06 [PMID: 36476859]
H Lin, SQ Ye, ZH Xu, JS Penaloza, M Aljuhani, T Vetter, MT Zhao, KL McBride Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD) Stem Cell Research, 2022-10-31;65(0):102958. 2022-10-31 [PMID: 36343514] (ICC, Human) ICC Human
István Fodor, Tamar Schwarz, Bence Kiss, Antal Tapodi, János Schmidt, Alex R. O. Cousins et al. Studies on a widely-recognized snail model species (Lymnaea stagnalis) provide further evidence that vertebrate steroids do not have a hormonal role in the reproduction of mollusks Front Endocrinol (Lausanne) 2022-09-08 [PMID: 36157463]
Jinrong Peng, Yao Xiao, Qian Yang, Qingya Liu, Yu Chen, Kun Shi et al. Intracellular aggregation of peptide-reprogrammed small molecule nanoassemblies enhances cancer chemotherapy and combinatorial immunotherapy Acta Pharmaceutica Sinica B 2021-04-01 [PMID: 33996418]
P Mergenthal, S Hariharan, JM Pemberton, C Lourenco, LZ Penn, DW Andrews Rapid 3D phenotypic analysis of neurons and organoids using data-driven cell segmentation-free machine learning PLoS computational biology, 2021-02-22;17(2):e1008630. 2021-02-22 [PMID: 33617523] (ICC, Human) ICC Human
LA Alves Avel, C Schrenk, M Sönnichsen, A Hamacher, FK Hansen, J Schliehe-D, A Borkhardt, S Bhatia, MU Kassack, T Kurz Synergistic induction of apoptosis in resistant head and neck carcinoma and leukemia by alkoxyamide-based histone deacetylase inhibitors European Journal of Medicinal Chemistry, 2020-12-17;211(0):113095. 2020-12-17 [PMID: 33360560] (Cell Culture, Human) Cell Culture Human
G Kaufman, D Skrtic N-Acetyl Cysteine Modulates the Inflammatory and Oxidative Stress Responses of Rescued Growth-Arrested Dental Pulp Microtissues Exposed to TEGDMA in ECM Int J Mol Sci, 2020-10-03;21(19):. 2020-10-03 [PMID: 33023018] (ICC, Human) ICC Human
Dhananjay V. Deshmukh, Nils Pasquero, Gajraj Rathore, Joel Zvick, Ori Bar‐Nur, Jurg Dual et al. Screening method to identify hydrogel formulations that facilitate myotube formation from encapsulated primary myoblasts Bioengineering & Translational Medicine 2020-09-03 [PMID: 33005743]
Show All 30 Publications.

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Product General Protocols

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FAQs for IgG Antibody (NL007) (0)

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Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHG1