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Donkey anti-Mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

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Western blot shows detection of Mouse Anti-Human/Mouse/Rat IGSF4C/SynCAM4 Monoclonal Antibody (Catalog # MAB41642) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). This ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP (Horseradish Peroxidase)

Order Details

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Catalog# & Conjugate Size Price
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Donkey anti-Mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary

Immunogen
Mouse IgG
Specificity
Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with human IgG, rabbit IgG and chicken IgY is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Western Blot
Reviewed Applications
Read 1 Review rated 5
using
HAF018 in the following application:

Publications
Read Publications using
HAF018 in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied as a 0.2 μm filtered solution in PBS with stabilizers.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (HAF018)(29)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 3 applications: ELISA Capture, Functional Assay, Western Blot.


Filter By Application
ELISA Capture
(1)
Functional Assay
(1)
Western Blot
(11)
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Filter By Species
Human
(8)
Mouse
(4)
Rat
(1)
N/A
(1)
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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using HAF018 Applications Species
Nakanishi, R;Hashimoto, N;Takuwa, M;Xing, J;Uemura, M;Un Nisa, B;Tanaka, M;Hirabayashi, T;Tanaka, M;Fujino, H; High Concentrations of Nucleotides Prevent Capillary Regression during Hindlimb Unloading by Inhibiting Oxidative Stress and Enhancing Mitochondrial Metabolism of Soleus Muscles in Rats Acta histochemica et cytochemica 2023-12-28 [PMID: 38318105]
Nakanishi, R;Tanaka, M;Nisa, BU;Shimizu, S;Hirabayashi, T;Tanaka, M;Maeshige, N;Roy, RR;Fujino, H; Alternating current electromagnetic field exposure lessens intramyocellular lipid accumulation due to high-fat feeding via enhanced lipid metabolism in mice PloS one 2023-11-27 [PMID: 38011220] (Mouse) Mouse
Manish Mishra, Manisha Yadav, Sandeep Kumar, Raj Kumar, Pradip Sen TIM-3 increases the abundance of type-2 dendritic cells during Leishmania donovani infection by enhancing IL-10 production via STAT3 Cell Death & Disease 5/18/2023 [PMID: 37202419]
Kanokwan Sriwattanapong, Thanakorn Theerapanon, Lawan Boonprakong, Anucharte Srijunbarl, Thantrira Porntaveetus, Vorasuk Shotelersuk Novel ITGB6 variants cause hypoplastic-hypomineralized amelogenesis imperfecta and taurodontism: characterization of tooth phenotype and review of literature BDJ Open 4/11/2023 [PMID: 37041139]
Rahul Mallick, Erika Gurzeler, Pyry I. Toivanen, Tiina Nieminen, Seppo Ylä-Herttuala Novel Designed Proteolytically Resistant VEGF-B186R127S Promotes Angiogenesis in Mouse Heart by Recruiting Endothelial Progenitor Cells Frontiers in Bioengineering and Biotechnology 8/4/2022 [PMID: 35992336]
MN Akhtar, S Kumar, P Sen Leishmania donovani Impedes Antileishmanial Immunity by Suppressing Dendritic Cells via the TIM-3 Receptor MBio, 2022-08-04;13(4):e0330921. 2022-08-04 [PMID: 35924848] (Western Blot, Mouse) Western Blot Mouse
Mai M. Abdelmoaty, Jatin Machhi, Pravin Yeapuri, Farah Shahjin, Vikas Kumar, Katherine E. Olson et al. Monocyte biomarkers define sargramostim treatment outcomes for Parkinson's disease Clinical and Translational Medicine 7/8/2022 [PMID: 35802825]
Barrett P, Quick TJ, Mudera V, Player DJ. Neuregulin 1 Drives Morphological and Phenotypical Changes in C2C12 Myotubes: Towards De Novo Formation of Intrafusal Fibres In Vitro Frontiers in Cell and Developmental Biology 2022-01-11 [PMID: 35087826]
Barrett P, Quick TJ, Mudera V, Player DJ. Neuregulin 1 Drives Morphological and Phenotypical Changes in C2C12 Myotubes: Towards De Novo Formation of Intrafusal Fibres In Vitro Frontiers in Cell and Developmental Biology 1/11/2022 [PMID: 35087826]
Henna Korpela, Olli-Pekka Hätinen, Tiina Nieminen, Rahul Mallick, Pyry Toivanen, Jonna Airaksinen et al. Adenoviral VEGF-B186R127S gene transfer induces angiogenesis and improves perfusion in ischemic heart iScience 12/17/2021 [PMID: 34917905]
Show All 29 Publications.

Review for IgG Antibody (HAF018) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Pig.

Reviews using HAF018:
Filter by Applications
WB
(1)
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Filter by Species
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot IgG HAF018
Enlarge
5
reviewed by:
Verified Customer
WB Pig 08/14/2019
View

Summary

ApplicationWestern Blot
Sample TestedMuscle tissue
SpeciesPig
LotWVA091611

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IgG Antibody (HAF018) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Recent Reviews

5
5
1
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1
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Verified Customer
08/14/2019
Application: WB
Species: Pig

Bioinformatics

Gene Symbol IGHG1