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Recombinant Human IGF-II/IGF2 Protein

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SDS-Page: Recombinant Human IGF-II Protein [NBP2-59722] - Recombinant IGF-II Protein [NBP2-59722] - 98 % by SDS-PAGE and HPLC analyses.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Catalog# & Formulation Size Price
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Recombinant Human IGF-II/IGF2 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 67 amino acids corresponding to IGF-II/IGF2 Source: E. coli

Uniprot ID: E3UN46

Amino Acid Sequence: AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

Details of Functionality
IGF-II/IGF2 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IGF2
Purity
> 98 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of IGF-II/IGF2 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
7.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 0.02 % Tween-20.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 98 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human IGF-II/IGF2 Protein

  • C11orf43
  • chromosome 11 open reading frame 43
  • FLJ22066
  • FLJ44734
  • GRDF
  • IGF2
  • IGF-2
  • IGFII
  • IGF-II
  • insulin-like growth factor 2 (somatomedin A)
  • insulin-like growth factor II
  • insulin-like growth factor type 2
  • MSA
  • PEG2
  • PP9974
  • somatomedin-A

Background

Belongs to the insulin family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IGF2