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IGF-II/IGF2 Antibody (75015) [Unconjugated]

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Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IGF-II/IGF2 (14 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB292). The ND50 is typically 2-12 µg/mL.
Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications B/N
Clone
75015
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB292-SP
Carrier Free
MAB292-500
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IGF-II/IGF2 Antibody (75015) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IGF-II/IGF2
Ala25-Glu91
Accession # P01344.1
Specificity
Detects human IGF-II/IGF2 in direct ELISA and Western blots. This antibody shows 100% cross-reactivity with recombinant mouse (rm) IGF‑II and no cross-reactivity with recombinant human IGF-I or rmIGF-I.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IGF2
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 2-12 ug/mL
Publications
Read Publications using
MAB292 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-II/IGF2 Antibody (75015) [Unconjugated]

  • C11orf43
  • chromosome 11 open reading frame 43
  • FLJ22066
  • FLJ44734
  • GRDF
  • IGF2
  • IGF-2
  • IGFII
  • IGF-II
  • insulin-like growth factor 2 (somatomedin A)
  • insulin-like growth factor II
  • insulin-like growth factor type 2
  • MSA
  • PEG2
  • PP9974
  • somatomedin-A

Background

Insulin-like growth factor I (also known as somatomedin C and somatomedin A) and insulin-like growth factor II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved (100% identity between human, bovine, and porcine proteins) and exhibit cross-species activity.

IGF-II is a potent mitogenic growth factor. However, unlike IGF-I which has important postnatal roles, the growth-promoting function of IGF-II is limited to embryonic development.

Two specific cell surface receptors that bind IGF-I and IGF-II have been identified. The type I IGF receptor that participates in IGF signaling is structurally related to the insulin receptor. It is a disulfide-linked heterotetrameric transmembrane glycoprotein with an intracellular tyrosine kinase domain. Type I IGF receptor binds IGF-I with higher affinity than IGF-II. The type II IGF receptor which binds IGF-II with much higher affinity than IGF-I is also the cation-independent mannose 6-phosphate receptor. At the present time, it is not known if the type II IGF receptor participates in the IGF signaling pathway. An additional unknown receptor which mediates IGF‑II signaling has also been proposed. Circulating IGFs exist in complexes bound to IGF binding proteins. Currently, at least six high affinity binding proteins have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IGF-II/IGF2 Antibody (MAB292)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ELISA Development, ELISA Standard, Neutralization.


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ELISA Development
(1)
ELISA Standard
(1)
Neutralization
(2)
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Human
(3)
Mouse
(1)
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Showing Publications 1 - 8 of 8.
Publications using MAB292 Applications Species
F Kano, N Hashimoto, Y Liu, L Xia, T Nishihara, W Oki, K Kawarabaya, N Mizusawa, K Aota, T Sakai, M Azuma, H Hibi, T Iwasaki, T Iwamoto, N Horimai, A Yamamoto Therapeutic benefits of factors derived from stem cells from human exfoliated deciduous teeth for radiation-induced mouse xerostomia Scientific Reports, 2023-02-15;13(1):2706. 2/15/2023 [PMID: 36792628]
C De Giovann, P Nanni, L Landuzzi, ML Ianzano, G Nicoletti, S Croci, A Palladini, PL Lollini Immune targeting of autocrine IGF2 hampers rhabdomyosarcoma growth and metastasis BMC Cancer, 2019-02-07;19(1):126. 2019-02-07 [PMID: 30732578] (ELISA Standard, Mouse) ELISA Standard Mouse
SP Guan, ATL Lam, JP Newman, KLM Chua, CYL Kok, ST Chong, MLK Chua, PYP Lam Matrix metalloproteinase-1 facilitates MSC migration via cleavage of IGF-2/IGFBP2 complex FEBS Open Bio, 2017-11-23;8(1):15-26. 2017-11-23 [PMID: 29321953] (Neutralization, Human) Neutralization Human
Amelie Forest, Michael Amatulli, Dale L. Ludwig, Christopher B. Damoci, Ying Wang, Colleen A. Burns et al. Intrinsic Resistance to Cixutumumab Is Conferred by Distinct Isoforms of the Insulin Receptor Molecular Cancer Research 12/1/2015 [PMID: 26263910]
Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells. Br J Cancer, 2014-05-22;110(12):2865-73. 2014-05-22 [PMID: 24853185] (Neutralization, Human) Neutralization Human
Travis B. Salisbury, Gary Z. Morris, Justin K. Tomblin, Ateeq R. Chaudhry, Carla R. Cook, Nalini Santanam Aryl Hydrocarbon Receptor Ligands Inhibit IGF-II and Adipokine Stimulated Breast Cancer Cell Proliferation ISRN Endocrinology 9/23/2013 [PMID: 24171117]
Y. Zhang, F. Jalili, N. Ouamara, A. Zameer, G. Cosentino, M. Mayne et al. Glatiramer acetate-reactive T lymphocytes regulate oligodendrocyte progenitor cell number in vitro: Role of IGF-2 Journal of Neuroimmunology 10/8/2010 [PMID: 20637510]
Adkins RM, Fain JN, Krushkal J Association between paternally inherited haplotypes upstream of the insulin gene and umbilical cord IGF-II levels. Pediatr. Res., 2007-10-01;62(4):451-5. 2007-10-01 [PMID: 17667841] (ELISA Development, Human) ELISA Development Human

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Bioinformatics

Gene Symbol IGF2
Uniprot