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IFN-alpha I/IFNA17 Antibody (995826) [Unconjugated]

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Recombinant Human IFN- alpha I reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN- alpha I (20 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN- alpha I Monoclonal Antibody (Catalog # MAB10080). The ND50 is typically 0.6-6 ng/mL.
Recombinant Human IFN- alpha I reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications B/N
Clone
995826
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB10080-SP
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IFN-alpha I/IFNA17 Antibody (995826) [Unconjugated] Summary

Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human IFN‑ alpha I
Met1-Asp189
Accession # P01571
Specificity
Detects human IFN‑ alpha I in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Neutralization 0.6-6 ng/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha I/IFNA17 Antibody (995826) [Unconjugated]

  • IFNA
  • IFNA17
  • IFNalpha I
  • IFN-alpha I
  • IFN-alpha-17
  • IFN-alphaI
  • INFA
  • interferon alpha-17
  • Interferon alpha-88
  • Interferon alpha-I'
  • Interferon alpha-T
  • interferon alpha-WA
  • interferon, alpha 17
  • LeIF I
  • LEIF2C1

Background

Interferon-alpha (IFN-alpha, IFNA), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. There are at least 18 different IFNA subtypes, and the major subtypes identified are IFNA1, IFNA2, IFNA8, IFNA10, IFNA14 and IFNA21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IFN-alpha I/IFNA17
Antibody (995826) [Un ...
MAB10080
IFN-alpha I/IFNA17 Antibody (995826) [Unconjugated]
Species: Hu
Applications: B/N

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