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ICOS Antibody (669222) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
669222
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB6975U-100UG
Alexa Fluor 405
FAB6975V-100UG
Alexa Fluor 488
FAB6975G-100UG
Alexa Fluor 532
FAB6975X
Alexa Fluor 594
FAB6975T-100UG
Alexa Fluor 647
FAB6975R-100UG
Alexa Fluor 700
FAB6975N-100UG
Alexa Fluor 750
FAB6975S-100UG
Allophycocyanin
FAB6975A
Allophycocyanin/Cy7
FAB6975APCCY7
Biotin
FAB6975B
DyLight 350
FAB6975D
DyLight 405
FAB6975E
DyLight 488
FAB6975K
DyLight 550
FAB6975L
DyLight 594
FAB6975M
DyLight 650
FAB6975W
DyLight 680
FAB6975Y
DyLight 755
FAB6975Z
FITC
FAB6975F
HRP
FAB6975H
Janelia Fluor 525
FAB6975JF525
Janelia Fluor 549
FAB6975I
Janelia Fluor 585
FAB6975JF585
Janelia Fluor 635
FAB6975JF635
Janelia Fluor 646
FAB6975J
Janelia Fluor 669
FAB6975JF669
PE/Atto594
FAB6975PEATT594
PE/Cy5.5
FAB6975PECY55
PE/Cy7
FAB6975PECY7
PerCP
FAB6975C
Phycoerythrin
FAB6975P
Unconjugated
MAB6975
mFluor Violet 450 SE
FAB6975MFV450
mFluor Violet 500 SE
FAB6975MFV500
mFluor Violet 610 SE
FAB6975MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB6975
Carrier Free
MAB6975
Carrier Free
FAB6975A
Carrier Free
FAB6975P
Carrier Free
MAB6975-SP
Carrier Free
FAB6975V-100UG
Carrier Free
FAB6975U-100UG
Carrier Free
FAB6975T-100UG
Carrier Free
FAB6975S-100UG
Carrier Free
FAB6975R-100UG
Carrier Free
FAB6975N-100UG
Carrier Free
FAB6975G-100UG
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ICOS Antibody (669222) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
NS0 mouse myeloma cell line transfected with human ICOS and mouse myeloma cell line NS0-derived recombinant human ICOS cocktail
Glu21-Phe141
Accession # Q9Y6W8
Specificity
Detects human ICOS in direct ELISAs.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ICOS
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for ICOS Antibody (669222) [CoraFluor™ 1]

  • Activation-inducible lymphocyte immunomediatory molecule
  • AILIM
  • AILIMCD278
  • CD278 antigen
  • CD278
  • CRP-1
  • CVID1
  • ICOS
  • inducible costimulator
  • inducible T-cell costimulator
  • inducible T-cell co-stimulator
  • MGC39850

Background

The C398.4A antibody reacts with the human, mouse and rat 47-57 kD ICOS protein, also known as inducible costimulatory molecule and H4. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ICOS