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ICAM-3/CD50 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF813
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF813
Carrier Free
AF813
Carrier Free
AF813-SP
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ICAM-3/CD50 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ICAM‑3/CD50
Gln30-His485
Accession # CAA49473
Specificity
Detects human ICAM‑3/CD50 in ELISAs and Western blots. In Western blot, approximately 2% cross‑reactivity with recombinant human PECAM is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ICAM3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Detection: Human ICAM-3/CD50 Biotinylated Antibody (Catalog # BAF813)
Standard: Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC)
Readout System
Publications
Read Publication using
BAF813 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-3/CD50 Antibody [Biotin]

  • CD50 antigen
  • CD50
  • CDW50CDw50
  • ICAM3
  • ICAM-3
  • ICAM-Rintercellular adhesion molecule-3
  • intercellular adhesion molecule 3

Background

Intercellular Adhesion Molecule-3 (ICAM-3; also known as CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha d/ beta 2. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.

  1. Hayflick, J. et al. (1998) Immunologic Res. 17:313.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ICAM-3/CD50 Antibody (BAF813)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Development.


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Bioinformatics

Gene Symbol ICAM3
Uniprot