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ICAM-3/CD50 Antibody (ICAM3/1019) [Allophycocyanin/Cy7]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
Clone
ICAM3/1019
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin/Cy7

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47903AF350
Alexa Fluor 405
NBP2-47903AF405
Alexa Fluor 488
NBP2-47903AF488
Alexa Fluor 532
NBP2-47903AF532
Alexa Fluor 594
NBP2-47903AF594
Alexa Fluor 647
NBP2-47903AF647
Alexa Fluor 700
NBP2-47903AF700
Alexa Fluor 750
NBP2-47903AF750
Allophycocyanin
NBP2-47903APC
Biotin
NBP2-47903B
CoraFluor 1
NBP2-47903CL1
DyLight 350
NBP2-47903UV
DyLight 405
NBP2-47903V
DyLight 488
NBP2-47903G
DyLight 550
NBP2-47903R
DyLight 594
NBP2-47903DL594
DyLight 650
NBP2-47903C
DyLight 680
NBP2-47903FR
DyLight 755
NBP2-47903IR
FITC
NBP2-47903F
HRP
NBP2-47903H
Janelia Fluor 525
NBP2-47903JF525
Janelia Fluor 549
NBP2-47903JF549
Janelia Fluor 585
NBP2-47903JF585
Janelia Fluor 635
NBP2-47903JF635
Janelia Fluor 646
NBP2-47903JF646
Janelia Fluor 669
NBP2-47903JF669
PE
NBP2-47903PE
PE/Atto594
NBP2-47903PEATT594
PE/Cy5.5
NBP2-47903PECY55
PE/Cy7
NBP2-47903PECY7
PerCP
NBP2-47903PCP
Unconjugated
NBP2-44550-0.1mg
mFluor Violet 450 SE
NBP2-47903MFV450
mFluor Violet 500 SE
NBP2-47903MFV500
mFluor Violet 610 SE
NBP2-47903MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44550-0.1mg
Azide and BSA Free
NBP2-47903-0.1mg
IHC-Prediluted
NBP2-48227
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ICAM-3/CD50 Antibody (ICAM3/1019) [Allophycocyanin/Cy7] Summary

Immunogen
Recombinant human ICAM-3/CD50 protein (Uniprot: P32942)
Localization
Cell surface
Specificity
Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This monoclonal antibody is excellent for staining of formalin/paraffin tissues.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ICAM3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for ICAM-3/CD50 Antibody (ICAM3/1019) [Allophycocyanin/Cy7]

  • CD50 antigen
  • CD50
  • CDW50CDw50
  • ICAM3
  • ICAM-3
  • ICAM-Rintercellular adhesion molecule-3
  • intercellular adhesion molecule 3

Background

All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM 3 is the major ligand for LFA-1 in the resting state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ICAM3