Novus Biologicals products are now on bio-techne.com

Human IFN-gamma Quantikine ELISA Kit

Product Details

Summary
Product Discontinued
View other related IFN-gamma ELISA Kits

Order Details


    • Catalog Number
      DIF50
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Human IFN-gamma Quantikine ELISA Kit Summary

Background
The Quantikine Human IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure IFN-gamma levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring human IFN-gamma samples showed linear curves that were parallel to the st...andard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.
Show More
Additional Information
This product has been discontinuation and replaced by catalog # DIF50C.
Specificity
Natural and recombinant human IFN-gamma
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IFNG

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
DIF50 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IFN-gamma Quantikine ELISA Kit

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Customers Who Viewed This Item Also Viewed...

6507-IL/CF
Species: Hu
Applications: BA
202-IL
Species: Hu
Applications: BA
DY417
Species: Mu
Applications: ELISA
M6000B
Species: Mu
Applications: ELISA
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
M5000
Species: Mu
Applications: ELISA
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
NBP2-75930
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
201-LB
Species: Hu
Applications: BA
7268-CT
Species: Hu
Applications: BA
NB300-605
Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB
DY413
Species: Mu
Applications: ELISA
7625
Species: Mu
Applications: ELISA
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NB600-1441
Species: Ca, Hu, Mu, Po
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KD
DIF50
Species: Hu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IFN-gamma (DIF50)(206)

We have publications tested in 6 confirmed species: Human, Mouse, Bovine, Cynomolgus Monkey, Primate, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 2 applications: Cell Culture, ELISA Capture.


Filter By Application
Cell Culture
(1)
ELISA Capture
(1)
All Applications
Filter By Species
Human
(200)
Mouse
(4)
Bovine
(1)
Cynomolgus Monkey
(1)
Primate
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 206. Show All 206 Publications.
Publications using DIF50 Applications Species
Kim, H;Park, HH;Kim, HN;Seo, D;Hong, KS;Jang, JG;Seo, EU;Kim, IY;Jeon, SY;Son, B;Cho, SW;Kim, W;Ahn, JH;Lee, W; The TOX-RAGE axis mediates inflammatory activation and lung injury in severe pulmonary infectious diseases Proceedings of the National Academy of Sciences of the United States of America 2024-06-25 [PMID: 38900789] (Human) Human
Ma?uj?o-Balcerska, E;Pietras, T; Deiodinase Types 1 and 3 and Proinflammatory Cytokine Values May Discriminate Depressive Disorder Patients from Healthy Controls Journal of clinical medicine 2023-09-24 [PMID: 37834806] (Human) Human
G Silva, AF Rodrigues, S Ferreira, C Matos, RP Eleutério, G Marques, K Kucheryava, AR Lemos, PMF Sousa, R Castro, A Barbas, D Simão, PM Alves Novel scFv against Notch Ligand JAG1 Suitable for Development of Cell Therapies toward JAG1-Positive Tumors Biomolecules, 2023-03-02;13(3):. 2023-03-02 [PMID: 36979394] (Human) Human
AG Saleh, AY Shehab, R Abdul-Ghan, AF Allam, MM Osman, HS Ibrahim, HA El-Taweel, EA Moneer, NA Hagras, HF Farag Key cytokines and hematological parameters in patients with uncomplicated falciparum malaria in Hodeidah, Yemen Cytokine, 2023-02-27;164(0):156156. 2023-02-27 [PMID: 36857851] (Human) Human
F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells bioRxiv : the preprint server for biology, 2023-02-13;0(0):. 2023-02-13 [PMID: 36789428] (Human) Human
SY Cho, SM Jeong, YJ Jeon, SJ Yang, JE Hwang, BM Yoo, HS Kim WT1 Pulsed Human CD141+ Dendritic Cell Vaccine Has High Potential in Solid Tumor-Targeted Immunotherapy International Journal of Molecular Sciences, 2023-01-12;24(2):. 2023-01-12 [PMID: 36675017] (Human) Human
MC Giménez, M Luxwolda, EG Van Stipri, PP Bollen, RL Hoekman, MA Koopmans, PR Arany, MR Krames, AC Berends, RA Hut, MCM Gordijn Effects of Near-Infrared Light on Well-Being and Health in Human Subjects with Mild Sleep-Related Complaints: A Double-Blind, Randomized, Placebo-Controlled Study Biology, 2022-12-29;12(1):. 2022-12-29 [PMID: 36671752] (Human) Human
A Barilli, R Visigalli, F Ferrari, G Recchia Lu, M Soli, V Dall'Asta, BM Rotoli Growth Arrest of Alveolar Cells in Response to Cytokines from Spike S1-Activated Macrophages: Role of IFN-gamma Biomedicines, 2022-12-01;10(12):. 2022-12-01 [PMID: 36551841] (Human) Human
P Yuti, Y Wutti-In, N Sawasdee, K Kongkhla, N Phanthapho, K Choomee, T Chieochans, A Panya, M Junking, PT Yenchitsom, J Sujjitjoon Anti-CD19 chimeric antigen receptor T cells secreting anti-PD-L1 single-chain variable fragment attenuate PD-L1 mediated T cell inhibition International immunopharmacology, 2022-11-23;113(0):109442. 2022-11-23 [PMID: 36435066] (Human) Human
CV Gonçalves, IS Ribeiro, MPL Galantini, IPR Muniz, PHB Lima, GS Santos, RAA da Silva Inflammaging and body composition: New insights in diabetic and hypertensive elderly men Experimental gerontology, 2022-10-28;170(0):112005. 2022-10-28 [PMID: 36341786] (Human) Human
Show All 206 Publications.

Reviews for IFN-gamma (DIF50) (0)

There are no reviews for IFN-gamma (DIF50). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IFN-gamma (DIF50). (Showing 1 - 1 of 1 FAQs).

  1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
    • We are research use only.

Additional IFN-gamma Products

Blogs on IFN-gamma.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

Review this Product

Be the first to review our Human IFN-gamma Quantikine ELISA Kit and receive a gift card or discount.

Bioinformatics

Gene Symbol IFNG