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HSP90 Antibody (S08-8A1)

Images

 
Western Blot: HSP90 Antibody (S08-8A1) [NBP3-15043] - Western blot detection of Hsp90 in Rat Brain, C6, 3T3, Hela cell lysates using Hsp90 Rabbit mAb (1:1000 diluted). Predicted band size: 85, 83kDa. Observed band size: ...read more
Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (S08-8A1) [NBP3-15043] - Immunofluorescence of Hsp90 (green) in Hela cells using NBP3-15043 at dilution 1/200, and DAPI (blue)
Immunohistochemistry-Paraffin: HSP90 Antibody (S08-8A1) [NBP3-15043] - Immunohistochemistry of HSP90 in paraffin-embedded Human tonsil using HSP90 Rabbit mAb at dilution 1/5

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
S08-8A1
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HSP90 Antibody (S08-8A1) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein of human HSP90 (Uniprot#: P07900 P08238)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
HSP90AA1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1/50
  • Immunohistochemistry 1/200
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000-1/10000
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Purity
Affinity purified

Alternate Names for HSP90 Antibody (S08-8A1)

  • FLJ31884
  • Heat shock 86 kDa
  • heat shock 90kD protein 1, alpha
  • heat shock 90kD protein 1, alpha-like 4
  • heat shock 90kD protein, alpha-like 4
  • heat shock 90kDa protein 1, alpha
  • heat shock protein 90kDa alpha (cytosolic), class A member 1
  • heat shock protein HSP 90-alpha
  • Hsp89
  • HSP90
  • HSP90AHSP86
  • HSP90N
  • HSPC1HSP 86
  • HSPCAHSP89A
  • HSPCAL1
  • HSPCAL4
  • HSPN
  • LAP2
  • Renal carcinoma antigen NY-REN-38

Background

Hsp90 has at least 2 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steroid receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSP90 Antibody (NBP3-15043) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP90 Antibody (NBP3-15043) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for HSP90 Antibody (NBP3-15043)

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HSP90 - an essential eukaryotic protein with implications for drug development
The heat-shock protein 90 (HSP90) family is a group of highly conserved molecular chaperones with important functions in protein folding and in signal transduction. The HSP90 protein structure is so well conserved that some HSP90 antibodies are rea...  Read full blog post.

Integrin beta 1 binding protein 2
ITGB1BP2 is a muscle-specific protein cloned by a rat created by Branccio's group in Italy that was found to interact with the cytoplasmic domain of integrin beta 11. It is expressed only in heart and skeletal muscle but is not essential for normal de...  Read full blog post.

HSP Antibodies: Novel Therapies for MMP-induced Metastatic Breast Cancer
The matrix metalloproteinases are zinc-dependent protease enzymes which interact with a range of ECM (extracellular matrix) proteins, and are activated by proteolytic cleavage. We at Novus Biologicals offer a wide range of top quality MMP reagents, in...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

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Bioinformatics

Gene Symbol HSP90AA1