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HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free

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Western Blot: HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free [NBP2-70905] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF-4 alpha.
Western Blot: HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free [NBP2-70905] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF-4 alpha.
Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free [NBP2-70905] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI2H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70905AF350
Alexa Fluor 405
NBP2-70905AF405
Alexa Fluor 488
NBP2-70905AF488
Alexa Fluor 532
NBP2-70905AF532
Alexa Fluor 594
NBP2-70905AF594
Alexa Fluor 647
NBP2-70905AF647
Alexa Fluor 700
NBP2-70905AF700
Alexa Fluor 750
NBP2-70905AF750
Allophycocyanin
NBP2-70905APC
Biotin
NBP2-70905B
CoraFluor 1
NBP2-70905CL1
DyLight 350
NBP2-70905UV
DyLight 405
NBP2-70905V
DyLight 488
NBP2-70905G
DyLight 550
NBP2-70905R
DyLight 594
NBP2-70905DL594
DyLight 650
NBP2-70905C
DyLight 680
NBP2-70905FR
DyLight 755
NBP2-70905IR
FITC
NBP2-70905F
HRP
NBP2-70905H
Janelia Fluor 525
NBP2-70905JF525
Janelia Fluor 549
NBP2-70905JF549
Janelia Fluor 646
NBP2-70905JF646
Janelia Fluor 669
NBP2-70905JF669
PE
NBP2-70905PE
PerCP
NBP2-70905PCP
Unconjugated
NBP2-45989
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45989
Novus Biologicals offers 100% Guarantee

HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-315 of human HNF4A(NP_849180) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HNF4A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free

  • FLJ39654
  • hepatic nuclear factor 4 alpha
  • hepatocyte nuclear factor 4, alpha
  • hepatocyte nuclear factor 4-alpha
  • HNF4 alpha
  • HNF-4 alpha
  • HNF4A
  • HNF4a7
  • HNF-4-alpha
  • HNF4alpha10/11/12
  • HNF4HNF4a8
  • MODY
  • MODY1
  • NR2A1
  • NR2A1HNF4a9
  • NR2A21
  • Nuclear receptor subfamily 2 group A member 1
  • TCF
  • TCF14
  • TCF-14
  • TCF14HNF4alpha
  • Transcription factor 14
  • Transcription factor HNF-4
  • transcription factor-14

Background

HNF4 alpha is encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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HNF-4 alpha/NR2A1 Antibody (OTI2H2) - Azide and BSA Free
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HNF4A