![Western Blot: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA2.](http://images.novusbio.com/fullsize/HNF-3-beta-FoxA2-Antibody-OTI3C10-Azide-and-BSA-Free-Western-Blot-NBP2-70904-img0002.jpg "Western Blot: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA2.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI3C10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 300-458 of human FOXA2 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FOXA2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 48.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FOXA2 |
![Immunocytochemistry/Immunofluorescence: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY FOXA2.](http://images.novusbio.com/fullsize/HNF-3-beta-FoxA2-Antibody-OTI3C10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70904-img0004.jpg "Immunocytochemistry/Immunofluorescence: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY FOXA2.")
![Immunohistochemistry: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - Staining of paraffin-embedded Carcinoma of liver using anti-FOXA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HNF-3-beta-FoxA2-Antibody-OTI3C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70904-img0006.jpg "Immunohistochemistry: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - Staining of paraffin-embedded Carcinoma of liver using anti-FOXA2 mouse monoclonal antibody.")
![Flow Cytometry: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - Staining of HepG2 cells using anti HNF-3 beta/FoxA2 mouse monoclonal antibody at 1:50 dilution.](http://images.novusbio.com/fullsize/HNF-3-beta-FoxA2-Antibody-OTI3C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70904-img0005.jpg "Flow Cytometry: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - Staining of HepG2 cells using anti HNF-3 beta/FoxA2 mouse monoclonal antibody at 1:50 dilution.")
![Western Blot: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti HNF-3 beta/FoxA2 monoclonal antibody.(1:200).](http://images.novusbio.com/fullsize/HNF-3-beta-FoxA2-Antibody-OTI3C10-Azide-and-BSA-Free-Western-Blot-NBP2-70904-img0001.jpg "Western Blot: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti HNF-3 beta/FoxA2 monoclonal antibody.(1:200).")
![Immunohistochemistry: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - Staining of paraffin-embedded lung using anti-FOXA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HNF-3-beta-FoxA2-Antibody-OTI3C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70904-img0007.jpg "Immunohistochemistry: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - Staining of paraffin-embedded lung using anti-FOXA2 mouse monoclonal antibody.")
![Flow Cytometry: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - HEK293T cells transfected with either pCMV6-ENTRY FOXA2.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-FOXA2 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/HNF-3-beta-FoxA2-Antibody-OTI3C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70904-img0003.jpg "Flow Cytometry: HNF-3 beta/FoxA2 Antibody (OTI3C10) - Azide and BSA Free [NBP2-70904] - HEK293T cells transfected with either pCMV6-ENTRY FOXA2.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-FOXA2 mouse monoclonal, and then analyzed by flow cytometry.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")