We are interested in custom labeling our antibodies to be able to integrate new markers into already existing multicolor FACS panels. We would be glad to receive pricing and general order information for anti-HLA-DRB1 and anti-HLA-DRB5. To estimate expenses for future antibody labeling orders it would be helpful to know on what basis (amount, type, etc.) the price is going to be calculated. Right now the antibodies are still in mouse ascites. Would it be possible to integrate the elution of the antibodies from the ascites before labeling as part of the service agreement?

Name: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [Janelia Fluor&#174; 646]
Brand: Novus Biologicals
SKU: NBP2-47672JF646
Price: 519 USD

Question

Accepted Answer

While we do not generally do this kind of custom purification unless it is for very large amounts of antibody, we do have a number of kits available that may be of use to you. Since an antibody that is still in ascites can not be labeled, it will need to be purified. A list of our kits available for this can be found at the following link: AbSelect. Once the antibodies are purified, they can be labeled with any number of different colors. A list of these kits for labeling can be found at the following link: Lightning-Link.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, PmSpecies Glossary
Applications	WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
Clone	LN-3 + HLA-DRB/1067
Clonality	Monoclonal
Host	Mouse
Conjugate	Janelia Fluor 646

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP2-47672AF350

Alexa Fluor 405 NBP2-47672AF405

Alexa Fluor 488 NBP2-47672AF488

Alexa Fluor 532 NBP2-47672AF532

Alexa Fluor 594 NBP2-47672AF594

Alexa Fluor 647 NBP2-47672AF647

Alexa Fluor 700 NBP2-47672AF700

Alexa Fluor 750 NBP2-47672AF750

Allophycocyanin NBP2-47672APC

Allophycocyanin/Cy7 NBP2-47672APCCY7

Biotin NBP2-47672B

DyLight 350 NBP2-47672UV

DyLight 405 NBP2-47672V

DyLight 488 NBP2-47672G

DyLight 550 NBP2-47672R

DyLight 594 NBP2-47672DL594

DyLight 650 NBP2-47672C

DyLight 680 NBP2-47672FR

DyLight 755 NBP2-47672IR

FITC NBP2-47672F

HRP NBP2-47672H

Janelia Fluor 549 NBP2-47672JF549

PE NBP2-47672PE

PE/Atto594 NBP2-47672PEATT594

PE/Cy5.5 NBP2-47672PECY55

PE/Cy7 NBP2-47672PECY7

PerCP NBP2-47672PCP

Unconjugated NBP2-45316-0.1mg

mFluor Violet 450 SE NBP2-47672MFV450

mFluor Violet 500 SE NBP2-47672MFV500

mFluor Violet 610 SE NBP2-47672MFV610

Catalog# & Formulation	Size	Price
Standard Formulation NBP2-45316-0.1mg

Azide and BSA Free NBP2-47672-0.1mg

IHC-Prediluted NBP2-48136

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-47672AF350
Alexa Fluor 405	NBP2-47672AF405
Alexa Fluor 488	NBP2-47672AF488
Alexa Fluor 532	NBP2-47672AF532
Alexa Fluor 594	NBP2-47672AF594
Alexa Fluor 647	NBP2-47672AF647
Alexa Fluor 700	NBP2-47672AF700
Alexa Fluor 750	NBP2-47672AF750
Allophycocyanin	NBP2-47672APC
Allophycocyanin/Cy7	NBP2-47672APCCY7
Biotin	NBP2-47672B
DyLight 350	NBP2-47672UV
DyLight 405	NBP2-47672V
DyLight 488	NBP2-47672G
DyLight 550	NBP2-47672R
DyLight 594	NBP2-47672DL594
DyLight 650	NBP2-47672C
DyLight 680	NBP2-47672FR
DyLight 755	NBP2-47672IR
FITC	NBP2-47672F
HRP	NBP2-47672H
Janelia Fluor 549	NBP2-47672JF549
PE	NBP2-47672PE
PE/Atto594	NBP2-47672PEATT594
PE/Cy5.5	NBP2-47672PECY55
PE/Cy7	NBP2-47672PECY7
PerCP	NBP2-47672PCP
Unconjugated	NBP2-45316-0.1mg
mFluor Violet 450 SE	NBP2-47672MFV450
mFluor Violet 500 SE	NBP2-47672MFV500
mFluor Violet 610 SE	NBP2-47672MFV610

HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [Janelia Fluor® 646]

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Immunogen	Activated human peripheral blood mononuclear cells (LN-3 & HLA-DRB/1067)
Localization	Cell surface
Specificity	This monoclonal antibody reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.
Isotype	IgG2b Kappa
Clonality	Monoclonal
Host	Mouse
Gene	HLA-DRB1
Purity	Protein A or G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	CyTOF-ready Flow Cytometry Immunocytochemistry/ Immunofluorescence Immunofluorescence Immunohistochemistry Immunohistochemistry-Paraffin Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Protein A or G purified