Histone H3 [Citrulline Arg17, Citrulline Arg2, Citrulline Arg8] Antibody

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bacteria, WB.

We have publications tested in 6 applications: ELISA, FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P.

Showing Publications 1 - 10 of 15. Show All 15 Publications. Collapse Publications.

Publications using NB100-57135	Applications	Species
Ma T, Tang Y, Wang T et al. Chronic pulmonary bacterial infection facilitates breast cancer lung metastasis by recruiting tumor-promoting MHCII(hi) neutrophils Signal Transduction and Targeted Therapy 2023-08-11 [PMID: 37563136] (ICC/IF)	ICC/IF
Sung, PS;Sun, CP;Tao, MH;Hsieh, SL; Inhibition of SARS-CoV-2-mediated thromboinflammation by CLEC2.Fc EMBO molecular medicine 2023-05-22 [PMID: 37211986] (IHC-P, Mouse)	IHC-P	Mouse
Cha MJ, Ha J, Lee H et al. Neutrophil Recruitment in Arterial Thrombus and Characteristics of Stroke Patients with Neutrophil-Rich Thrombus Yonsei medical journal 2022-11-01 [PMID: 36303310] (IHC-P, Mouse) Details: Dilution used in IHC 1:50	IHC-P	Mouse
Chen CJ, Wu CC, Chang CY et al. Metformin Mitigated Obesity-Driven Cancer Aggressiveness in Tumor-Bearing Mice International journal of molecular sciences 2022-08-15 [PMID: 36012397] (IHC-P, FLOW, ELISA, Mouse, Human) Details: Human samples were used in Flow, Mouse in IHC-P and ELISA	IHC-P, FLOW, ELISA	Mouse, Human
Jin J, Qiao S, Liu J et al. Neutrophil extracellular traps promote thrombogenicity in cerebral venous sinus thrombosis Cell & bioscience 2022-07-22 [PMID: 35869501] (ICC/IF, Human) Details: 1ug/ml used	ICC/IF	Human
Sung PS, Yang SP, Peng YC et al. CLEC5A and TLR2 are critical in SARS-CoV-2-induced NET formation and lung inflammation Journal of biomedical science 2022-07-11 [PMID: 35820906] (ICC/IF, IHC-P, Mouse, Human)	ICC/IF, IHC-P	Mouse, Human
Kostyunin A, Glushkova T, Stasev A et al. Early Postoperative Immunothrombosis of Bioprosthetic Mitral Valve and Left Atrium: A Case Report International journal of molecular sciences 2022-06-16 [PMID: 35743174] (IF/IHC, Human)	IF/IHC	Human
Sun R, Wang X, Nie Y et al. Targeted trapping of endogenous endothelial progenitor cells for myocardial ischemic injury repair through neutrophil-mediated SPIO nanoparticle-conjugated CD34 antibody delivery and imaging Acta biomaterialia 2022-05-08 [PMID: 35545187] (ICC/IF, Mouse)	ICC/IF	Mouse
Safarulla S, Madan A, Xing F, Chandrasekaran A CXCR2 Mediates Distinct Neutrophil Behavior in Brain Metastatic Breast Tumor Cancers 2022-01-20 [PMID: 35158784] (ICC/IF, Human)	ICC/IF	Human
Zhang S, Cao Y, Du J Et al. Neutrophil extracellular traps contribute to tissue plasminogen activator resistance in acute ischemic stroke FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-09-01 [PMID: 34449927] (IF/IHC, ICC/IF, Human)	IF/IHC, ICC/IF	Human
Melo Z, Gutierrez-Mercado YK, Garcia-Martinez D et al. Sex-dependent mechanisms involved in renal tolerance to ischemia-reperfusion: Role of inflammation and histone H3 citrullination Transpl. Immunol. 2020-09-02 [PMID: 32890741] (IHC-Fr, Rat)	IHC-Fr	Rat
Callahan S, Doster R, Kelley BR et al. Induction of neutrophil extracellular traps (NETs) by Campylobacter jejuni Cell. Microbiol. 2020-04-23 [PMID: 32329205] (FLOW, Bacteria, WB)	FLOW	Bacteria, WB
Sung PS, Huang TF, Hsieh SL Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A/TLR2 Nat Commun. 2019-06-03 [PMID: 31160588] (IF/IHC, Mouse)	IF/IHC	Mouse
Raijmakers R et al. Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro. J Mol Biol;367(4):1118-29. 2007-01-01 [PMID: 17303166]
Mastronardi FG et al. Increased citrullination of histone H3 in multiple sclerosis brain and nimal models of demyelination: a role for tumor necrosis factor-induced peptidylarginine deiminase 4 translocation. J Neurosci 26:11387-96. 2006-01-01 [PMID: 17079667]
Show All 15 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	WB	Human and Mouse	11/25/2017	View Summary Application Western Blot Sample Tested mouse hepatocytes and HepG2 cells Species Human and Mouse Lot 16H8
Enlarge	4 reviewed by: Verified Customer	WB	Human	08/06/2016	View Summary Application Western Blot Species Human Lot PO number: E0520256

Array NB100-57135

• Histone H3 Antibodies
• Histone H3 Proteins