Histone H3

Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family members - histone H1, H2A, H2B, H3, and H4. Histone 3 is the most extensively modified of all histone family members and plays a key role in both short-term dynamic and long-term gene regulation.

Histone H3 antibody was used to determine if altered EID1 expression and nuclear localization may play a key role in the impaired synaptic plasticity and memory loss of Alzheimer’s Disease (1). In mammalian cell-fate regulation studies from the Salk Institute, Histone H3 antibody monitoring of modification states allowed researchers to establish that embryonic stem cells cycle in and out of a transient pluripotent potency state, possibly through embedded endogenous retroviral foreign sequences (2). Histone H3 antibody allowed the identification of SENP7 as a tumor suppressor in breast cancer epithelium (3).

The availability of DNA for processes such as replication, transcription, or repair is dictated by the dynamic changes that chromatin undergoes. These include rapid and numerous conformational changes such as winding and unwinding, as well as post-translational or “epigenetic” modifications such as methylation, acetylation, and phosphorylation. Epi-Plus antibodies targeted to specific histone modifications have helped advanced researchers understanding of key epigenetic pathways.

Dot Blot: Histone H3 [Trimethyl Lys9] Antibody