![Western Blot: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-1B3F12-A9-Western-Blot-NBP2-54770-img0001.jpg "Western Blot: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]")
| Reactivity | Hu, Y, YeSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, ChIP |
| Clone | 1B3F12/A9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Clone formerly called "H2BK123" |
| Immunogen | Ub-polypeptide antigen based on amino acids 115 - 130 of yeast Histone H2B |
| Modification | ubiquitylated Lys123 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HIST2H2BF |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Positive control(s): Yeast histone preparation. |
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| Theoretical MW | 23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publication using NBP2-54770 | Applications | Species |
|---|---|---|
| Vlaming et al. Direct screening for chromatin status on DNA barcodes in yeast delineates the regulome of H3K79 methylation by Dot1. elife. 2016-12-07 [PMID: 27922451] (Yeast) | Yeast |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H2B Antibody (NBP2-54770)Find related products by research area.
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| Gene Symbol | HIST2H2BF |
![Chromatin Immunoprecipitation: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-1B3F12-A9-Chromatin-Immunoprecipitation-ChIP-NBP2-54770-img0004.jpg "Chromatin Immunoprecipitation: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")