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Histone H2B [ubiquitylated Lys123] Antibody (1B3F12/A9) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, Y, YeSpecies Glossary
Applications WB, ELISA, ICC/IF, ChIP
Clone
1B3F12/A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

Histone H2B [ubiquitylated Lys123] Antibody (1B3F12/A9) [Janelia Fluor® 646] Summary

Description
Clone formerly called "H2BK123"
Immunogen
Ub-polypeptide antigen based on amino acids 115 - 130 of yeast Histone H2B
Modification
ubiquitylated Lys123
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HIST2H2BF
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined. Positive control(s): Yeast histone preparation.

Reactivity Notes

Saccharomyces cerevisiae

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Histone H2B [ubiquitylated Lys123] Antibody (1B3F12/A9) [Janelia Fluor® 646]

  • FLJ35099
  • FLJ56780
  • FLJ56787
  • HIST1H2BK
  • histone 2, H2bf
  • histone cluster 2, H2bf
  • histone H2B type 2-F
  • Histone H2B
  • MGC131639

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility tothe cellular machineries which require DNA as a template. Histones thereby play a central role in transcriptionregulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set ofpost-translational modifications of histones, also called histone code, and nucleosome remodeling

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HIST2H2BF