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HIST1H2BC Antibody (CL5048)

Images

 
Western Blot: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Purified human recombinant histone H2B, H2A, H3 and H4.
Immunohistochemistry: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Staining of human kidney shows strong nuclear positivity in renal tubules and in glomerular cells.
Western Blot: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Western blot analysis in human cell line HEL.
Western Blot: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Human cell line HEL.
Western Blot: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Staining of human prostate shows strong nuclear immunoreactivity in glandular and smooth muscle cells.
Immunohistochemistry: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Staining of human fallopian tube shows strong nuclear immunoreactivity in glandular and stromal cells.
Immunohistochemistry: HIST1H2BC Antibody (CL5048) [NBP2-61153] - Staining of human duodenum shows strong nuclear positivity in glandular cells and in lamina propria cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
CL5048
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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HIST1H2BC Antibody (CL5048) Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to the following amino acid sequence:VSEGTKAVTKYTSSK
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HIST1H2BC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20000 - 1:50000
  • Immunohistochemistry-Paraffin 1:20000 - 1:50000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for HIST1H2BC Antibody (CL5048)

  • H2B histone family, member K
  • H2B/a
  • H2B/g
  • H2B/kH2B/h
  • H2BFA
  • H2BFG
  • H2BFH
  • H2BFKH2B/l
  • H2BFL
  • HIST1H2BC
  • HIST1H2BE
  • HIST1H2BF
  • HIST1H2BG
  • histone 1, H2bi
  • histone cluster 1, H2bi
  • histone H2B type 1-C/E/F/G/I
  • Histone H2B.1 A
  • Histone H2B.a
  • Histone H2B.g
  • Histone H2B.h
  • Histone H2B.k
  • Histone H2B.l

Background

HIST1H2BC is 126 amino acids long, weighs approximately 14 kDa, and the gene codes for a part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, hemochromatosis, and immunodeficiency. HIST1H2BC has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the histone modification, signal transduction of Activin A, telomere maintenance, nucleosome assembly, cell cycle, and systemic lupus erythematosus pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HIST1H2BC