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HID1 Antibody

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Orthogonal Strategies: Western Blot: HID1 Antibody [NBP1-84075] - Analysis in human cell lines MCF-7 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunohistochemistry-Paraffin: HID1 Antibody [NBP1-84075] - Staining of human esophagus shows strong cytoplasmic positivity in superficial squamous epithelial cells.
Western Blot: HID1 Antibody [NBP1-84075] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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HID1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RPSTSSASGQWSPTPEWVLSWKSKLPLQTIMRLLQVLVPQVEKICIDKGLTDESEILRFLQHGTLVGLLPVPHPILIRKYQANSGTAM
Predicted Species
Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HID1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
HID1 Protein (NBP1-84075PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HID1 Antibody

  • C17orf28
  • chromosome 17 open reading frame 28
  • DMC1
  • DMC1FLJ43526
  • downregulated in multiple cancer 1
  • Down-regulated in multiple cancers 1
  • HID-1
  • hypothetical protein LOC283987

Background

HID1 may play an important role in the development of cancers in a broad range of tissues

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HID1