![Western Blot: HES-1 Antibody [NBP1-30912] - PC-12 whole cell extract.12 % SDS-PAGE HES1 antibody [N1], N-term dilution: 1:1000](http://images.novusbio.com/fullsize/HES-1-Antibody-Western-Blot-NBP1-30912-img0012.jpg "Western Blot: HES-1 Antibody [NBP1-30912] - PC-12 whole cell extract.12 % SDS-PAGE HES1 antibody [N1], N-term dilution: 1:1000")
| Reactivity | Hu, Mu, Rt, Po, BvSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HES-1. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Porcine (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HES1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
| Publication using NBP1-30912 | Applications | Species |
|---|---|---|
| Kang H, Yang K, Xiao L et al. Osteoblast Hypoxia-Inducible Factor-1a Pathway Activation Restrains Osteoclastogenesis via the Interleukin-33-MicroRNA-34a-Notch1 Pathway. Front Immunol. 2017-10-31 [PMID: 29085370] (WB, Mouse) | WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for HES-1 Antibody (NBP1-30912)Find related products by research area.
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| Gene Symbol | HES1 |
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody [NBP1-30912] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HES1 protein stained by HES1 antibody [N1], N-term diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HES-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30912-img0014.jpg "Immunocytochemistry/Immunofluorescence: HES-1 Antibody [NBP1-30912] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HES1 protein stained by HES1 antibody [N1], N-term diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Hoechst 33342 staining.")
![Immunoprecipitation: HES-1 Antibody [NBP1-30912] - A431 whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of HES1 by 2.5 ug of HES1 antibody 12% SDS-PAGE The immunoprecipitated HES1 protein was detected by HES1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HES-1-Antibody-Immunoprecipitation-NBP1-30912-img0011.jpg "Immunoprecipitation: HES-1 Antibody [NBP1-30912] - A431 whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of HES1 by 2.5 ug of HES1 antibody 12% SDS-PAGE The immunoprecipitated HES1 protein was detected by HES1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.")
![Western Blot: HES-1 Antibody [NBP1-30912] - MCF-7 whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HES-1 antibody [N1], N-term (NBP1-30912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30912_rabbit-polyclonal-hes-1-antibody-910202420104496.jpg "Western Blot: HES-1 Antibody [NBP1-30912] - MCF-7 whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HES-1 antibody [N1], N-term (NBP1-30912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: HES-1 Antibody [NBP1-30912] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-30912_rabbit-polyclonal-hes-1-antibody-1610202419405759.jpg "Western Blot: HES-1 Antibody [NBP1-30912] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")