Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-16749] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody diluted at 1:500. Blue: Hoechst 33342 ...read more
Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat duodenum. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: GRP78 Antibody [NBP2-16749] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.
Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract 7.5% SDS-PAGE Grp78 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody ...read more
Genetic Strategies: Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ...read more
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-16749] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody diluted at 1:500. Blue: Hoechst 33342 ...read more
Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat brain. Grp78 antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse brain. Grp78 antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse testis. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat liver. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse intestine. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - GRP78/HSPA5 proteins were measured by immunoblotting in epithelioid (EPI BAP1+ UPN7), biphasic (BIP BAP1- UPN16) and sarcomatous (BAP1+ UPN22) MPM cells. Tubulin was ...read more
72 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-16749 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified
Alternate Names for GRP78/HSPA5 Antibody
78 kDa glucose-regulated protein
BIP
Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
FLJ26106
GRP78
GRP-78
GRP78BIP
Heat shock 70 kDa protein 5
heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
HSP70-5
HSPA5
Immunoglobulin heavy chain-binding protein
MIF2
Background
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our GRP78/HSPA5 Antibody and receive a gift card or discount.