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GPT Antibody

Images

 
Western Blot: GPT Antibody [NBP2-16731] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: GPT Antibody [NBP2-16731] - Paraffin-embedded normal liver. GPT antibody [N2C2], Internal dilution: 1:500.

Product Details

Summary
Reactivity Hu, Bv, RMSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GPT Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GPT. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rhesus Macaque (97%), Bovine (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPT
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for GPT Antibody

  • AAT1alanine aminotransferase 1
  • ALT1
  • ALT1EC 2.6.1.2
  • Glutamate pyruvate transaminase 1
  • glutamic-alanine transaminase 1
  • Glutamic--alanine transaminase 1
  • glutamic-pyruvate transaminase (alanine aminotransferase)
  • Glutamic--pyruvic transaminase 1
  • GPT1GPT 1

Background

Glutamate-pyruvate transaminase (EC 2.6.1.2), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. It has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2; MIM 138210). See ALTQTL1 (MIM 612363) and ALTQTL2 (MIM 612364) for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol GPT
Uniprot