![Immunocytochemistry/Immunofluorescence: GPR81 Antibody [NLS2095] - LPS increases glycolysis as well as lactate production by BM neutrophils. MCT4, MCT1, and GPR81 (yellow) distribution on BM CD11b+ (green)/Ly6G+ (red) neutrophils visualized and quantified by ImageStream analysis. Images are from one representative experiment out of three. Scale bar indicates 7 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-020-17402-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunocytochemistry-Immunofluorescence-NLS2095-img0010.jpg "Immunocytochemistry/Immunofluorescence: GPR81 Antibody [NLS2095] - LPS increases glycolysis as well as lactate production by BM neutrophils. MCT4, MCT1, and GPR81 (yellow) distribution on BM CD11b+ (green)/Ly6G+ (red) neutrophils visualized and quantified by ImageStream analysis. Images are from one representative experiment out of three. Scale bar indicates 7 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-020-17402-2) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Product can be stored undiluted at 4C for up to 1 month. |
| Immunogen | Synthetic 19 amino acid peptide from C-terminus of human GPR81. |
| Epitope | C-Terminus |
| Localization | Multi-pass membrane protein |
| Specificity | Human GPR81. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except KRBA1 (53%). |
| Predicted Species | Primate (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HCAR1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB reactivity reported in (PMID: 19633298). ICC/IF reactivity reported in (PMID: 32071396). |
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| Publications |
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| Storage | Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for GPR81 Antibody (NLS2095)Find related products by research area.
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![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Brain, Substantia Nigra, pigmented and nonpigmented neurons.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0009.jpg "Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Brain, Substantia Nigra, pigmented and nonpigmented neurons.")
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Analysis of anti-FKSG80 / GPR81 antibody with human anterior pituitary.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0002.jpg "Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Analysis of anti-FKSG80 / GPR81 antibody with human anterior pituitary.")
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Analysis of anti-FKSG80 / GPR81 antibody with human colon, carcinoma.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0004.jpg "Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Analysis of anti-FKSG80 / GPR81 antibody with human colon, carcinoma.")
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human Skin, Melanoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0005.jpg "Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human Skin, Melanoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human Ovary, Carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0006.jpg "Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human Ovary, Carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human pancreas, carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0007.jpg "Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human pancreas, carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Breast, Carcinoma](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0008.jpg "Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Breast, Carcinoma")
![Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Stable knockdown of GPR81 in MDA-MB-231 cells. Using a lentiviral system, GPR81 was stably knocked down in MDA-MB-231 cells with GPR81-specific short hairpin RNA (shGPR81 #1) & compared with cells containing scrambled shRNA as a control (shNT). Knockdown of GPR81 was confirmed by (a) western blotting & (b) RT-qPCR. The GPR81, MCT1, & MCT4 mRNA levels in the shGPR81 #1 cells are presented as the fold increase compared with those in shNT cells. Data are presented as the means ± s.d. (n = 3/group). **p < 0.01; Student’s t-test. (c) Lactate production in shNT & shGPR81 #1 cells. The cells were cultured for 24 h & lactate concentrations in the supernatants were determined (n = 3/group). **p < 0.01 (vs. shNT cells); Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls2095_rabbit-gpr81-pab-bsa-free-310202415334917.jpg "Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Stable knockdown of GPR81 in MDA-MB-231 cells. Using a lentiviral system, GPR81 was stably knocked down in MDA-MB-231 cells with GPR81-specific short hairpin RNA (shGPR81 #1) & compared with cells containing scrambled shRNA as a control (shNT). Knockdown of GPR81 was confirmed by (a) western blotting & (b) RT-qPCR. The GPR81, MCT1, & MCT4 mRNA levels in the shGPR81 #1 cells are presented as the fold increase compared with those in shNT cells. Data are presented as the means ± s.d. (n = 3/group). **p < 0.01; Student’s t-test. (c) Lactate production in shNT & shGPR81 #1 cells. The cells were cultured for 24 h & lactate concentrations in the supernatants were determined (n = 3/group). **p < 0.01 (vs. shNT cells); Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Expression of GPR81 in breast cancer cells. (a) Western blot analysis of GPR81 in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). Cell lysates were analyzed by immunoblotting using anti-GPR81, anti-MCT4, anti-MCT1, & anti-beta -actin antibodies. (b) Immunocytochemical analysis of GPR81 expression in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). MCF-10A, MCF-7 & MDA-MB-231 cells were incubated with polyclonal antibodies against GPR81 followed by Alexa Fluor Plus 488-conjugated secondary antibody & visualized under a fluorescence microscope. Na/K ATPase was used as a cell membrane marker & the nuclei were stained with DAPI. Scale bar 10 μm. (c) MDA-MB-231 cells were cultured in the presence & absence of 20 mM lactate for 48 h, & cell lysates were analyzed by immunoblotting using anti-GPR81 & anti-beta -actin antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls2095_rabbit-gpr81-pab-bsa-free-31020241613628.jpg "Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Expression of GPR81 in breast cancer cells. (a) Western blot analysis of GPR81 in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). Cell lysates were analyzed by immunoblotting using anti-GPR81, anti-MCT4, anti-MCT1, & anti-beta -actin antibodies. (b) Immunocytochemical analysis of GPR81 expression in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). MCF-10A, MCF-7 & MDA-MB-231 cells were incubated with polyclonal antibodies against GPR81 followed by Alexa Fluor Plus 488-conjugated secondary antibody & visualized under a fluorescence microscope. Na/K ATPase was used as a cell membrane marker & the nuclei were stained with DAPI. Scale bar 10 μm. (c) MDA-MB-231 cells were cultured in the presence & absence of 20 mM lactate for 48 h, & cell lysates were analyzed by immunoblotting using anti-GPR81 & anti-beta -actin antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Expression of GPR81 in breast cancer cells. (a) Western blot analysis of GPR81 in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). Cell lysates were analyzed by immunoblotting using anti-GPR81, anti-MCT4, anti-MCT1, & anti-beta -actin antibodies. (b) Immunocytochemical analysis of GPR81 expression in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). MCF-10A, MCF-7 & MDA-MB-231 cells were incubated with polyclonal antibodies against GPR81 followed by Alexa Fluor Plus 488-conjugated secondary antibody & visualized under a fluorescence microscope. Na/K ATPase was used as a cell membrane marker & the nuclei were stained with DAPI. Scale bar 10 μm. (c) MDA-MB-231 cells were cultured in the presence & absence of 20 mM lactate for 48 h, & cell lysates were analyzed by immunoblotting using anti-GPR81 & anti-beta -actin antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls2095_rabbit-gpr81-pab-bsa-free-310202416114765.jpg "Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Expression of GPR81 in breast cancer cells. (a) Western blot analysis of GPR81 in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). Cell lysates were analyzed by immunoblotting using anti-GPR81, anti-MCT4, anti-MCT1, & anti-beta -actin antibodies. (b) Immunocytochemical analysis of GPR81 expression in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). MCF-10A, MCF-7 & MDA-MB-231 cells were incubated with polyclonal antibodies against GPR81 followed by Alexa Fluor Plus 488-conjugated secondary antibody & visualized under a fluorescence microscope. Na/K ATPase was used as a cell membrane marker & the nuclei were stained with DAPI. Scale bar 10 μm. (c) MDA-MB-231 cells were cultured in the presence & absence of 20 mM lactate for 48 h, & cell lysates were analyzed by immunoblotting using anti-GPR81 & anti-beta -actin antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")