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gp130/CD130 Antibody [Unconjugated]

 (11 Publications)
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Recombinant Human Oncostatin M/OSM (Catalog # 295-OM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human gp130 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-228-NA). The ND50 is typically 2‑10 µg/mL.
Recombinant Human Oncostatin M/OSM (Catalog # 295-OM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human ...read more
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human gp130 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-228-NA) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF228
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Catalog# & Formulation Size Price
Carrier Free
AF-228-SP
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gp130/CD130 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130
Leu24-Glu619 (Glu619Asp)
Accession # P40189
Specificity
Detects human gp130 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant rat gp130, recombinant mouse gp130, recombinant human (rh) IL-1 sRI, rhIL-1 sRII, rhIL-2 sR beta , rhIL-2 sR gamma , rhIL‑4 sR, and rhIL-6 sR is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL6ST
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.70 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 2-10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF-228-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for gp130/CD130 Antibody [Unconjugated]

  • CD130 antigen
  • CD130
  • CDw130
  • DKFZp564F053
  • gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
  • gp130
  • IL-6 receptor subunit beta
  • IL-6R subunit beta
  • IL-6RB
  • IL-6R-beta
  • IL6ST
  • interleukin 6 signal transducer (gp130, oncostatin M receptor)
  • interleukin receptor beta chain
  • interleukin-6 receptor subunit beta
  • Interleukin-6 signal transducer
  • Membrane glycoprotein 130
  • membrane glycoprotein gp130
  • Oncostatin-M receptor subunit alpha

Background

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. At the present time, the in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

  1. Narazaki, M. et al. (1993) Blood 82:1120.
  2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for gp130/CD130 Antibody (AF-228-NA)(11)

We have publications tested in 2 confirmed species: Human, Primate.

We have publications tested in 6 applications: Co-Immunoprecipitation, ICC, IHC-P, Neutralization, PLA, Western Blot.


Filter By Application
Co-Immunoprecipitation
(1)
ICC
(1)
IHC-P
(1)
Neutralization
(3)
PLA
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(10)
Primate
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF-228-NA Applications Species
Chandrasekar, B;Mummidi, S;DeMarco, VG;Higashi, Y; Empagliflozin Reverses Oxidized LDL-Induced RECK Suppression, Cardiotrophin-1 Expression, MMP Activation, and Human Aortic Smooth Muscle Cell Proliferation and Migration Mediators of inflammation 2023-10-04 [PMID: 37830075] (Western Blot, Human) Western Blot Human
SA Müller, MD Shmueli, X Feng, J Tüshaus, N Schumacher, R Clark, BE Smith, A Chi, S Rose-John, ME Kennedy, SF Lichtentha The Alzheimer&#039;s disease-linked protease BACE1 modulates neuronal IL-6 signaling through shedding of the receptor gp130 Molecular Neurodegeneration, 2023-02-21;18(1):13. 2023-02-21 [PMID: 36810097] (Western Blot, Primate) Western Blot Primate
P Filppu, JT Ramanathan, KJ Granberg, E Gucciardo, H Haapasalo, K Lehti, M Nykter, V Le Joncour, P Laakkonen CD109-GP130 interaction drives glioblastoma stem cell plasticity and chemoresistance through STAT3 activity JCI Insight, 2021-05-10;6(9):. 2021-05-10 [PMID: 33986188] (Co-Immunoprecipitation, PLA, Western Blot, Human) Co-Immunoprecipitation, PLA, Western Blot Human
Anderson J, Titz B, Akiyama R, Komisopoulou E, Park A, Tap W, Graeber T, Denny C Phosphoproteomic profiling reveals IL6-mediated paracrine signaling within the Ewing sarcoma family of tumors. Mol Cancer Res, 2014-08-04;12(12):1740-54. 2014-08-04 [PMID: 25092916] (Neutralization, Human) Neutralization Human
McFarland B, Hong S, Rajbhandari R, Twitty G, Gray G, Yu H, Benveniste E, Nozell S NF-kappaB-induced IL-6 ensures STAT3 activation and tumor aggressiveness in glioblastoma. PLoS ONE, 2013-11-11;8(11):e78728. 2013-11-11 [PMID: 24244348] (Neutralization, Human) Neutralization Human
Zhang Z, Lee JC Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer. Nat. Genet., 2012-07-01;44(8):852-860. 2012-07-01 [PMID: 22751098] (Western Blot, Human) Western Blot Human
Aghajanova L, Altmae S, Bjuresten K, Hovatta O, Landgren BM, Stavreus-Evers A Disturbances in the LIF pathway in the endometrium among women with unexplained infertility. Fertil. Steril., 2008-08-05;91(6):2602-10. 2008-08-05 [PMID: 18684446] (IHC-P, Human) IHC-P Human
Aghajanova L, Skottman H, Stromberg AM, Inzunza J, Lahesmaa R, Hovatta O Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells. Fertil. Steril., 2006-09-01;86(4):1193-209. 2006-09-01 [PMID: 16949591] (ICC, Human) ICC Human
Queen MM, Ryan RE, Holzer RG, Keller-Peck CR, Jorcyk CL Breast cancer cells stimulate neutrophils to produce oncostatin M: potential implications for tumor progression. Cancer Res., 2005-10-01;65(19):8896-904. 2005-10-01 [PMID: 16204061] (Neutralization, Human) Neutralization Human
He W, Gong K, Smith DK, Ip NY The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling. FEBS Lett., 2005-08-15;579(20):4317-23. 2005-08-15 [PMID: 16051226] (Western Blot, Human) Western Blot Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol IL6ST
Uniprot